LOCUS       sequence-285918-        2021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3125
FEATURES             Location/Qualifiers
     source          1..2021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1853..1877)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1885..1926
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgatgaagtc ccaggggtta
       61 gtatcattca aggatgtggc tgtggatttc acccaggagg agtggcagca acttgaccct
      121 tctcagagga ccctgtacag ggatgtgatg ctggagaact acagccacct ggtctcaatg
      181 gggtatccag tttccaaacc agatgtcatc tccaagttgg aacaaggaga agagccatgg
      241 atcataaagg gagacatatc aaattggatc tatccagatg aatatcaggc agatgggaga
      301 caagacagga agagtaacct tcacaactcc cagtcatgta ttttggggac agtttccttc
      361 catcataaga tactgaaagg agtcacaagg gatggttcat tgtgctccat tttaaaagtc
      421 tgtcaaggtg atggtcagct gcagagattt ctagagaatc aagacaaact cttcaggcag
      481 gtcacatttg ttaacagcaa aacagtgact gaggcatcag ggcataaata taatccactg
      541 gggaaaatat ttcaagagtg catagaaaca gatatatcaa tacagagatt ccataaatat
      601 gatgctttta aaaagaactt aaaaccaaat attgacctac cgagttgtta taagagcaat
      661 tcaagaaaaa aacctgatca gagttttgga ggtggaaaat catctagcca gagtgagccc
      721 aattctaatc ttgagaagat tcacaatgga gtaatacctt ttgatgataa tcagtgtgga
      781 aacgttttta gaaatacaca atcccttatt caatatcaga atgtggaaac taaagagaaa
      841 agctgtgtat gtgttacatg tggaaaagcc tttgctaaga agtcacaact cattgtacat
      901 caaagaattc atactggaaa gaaaccatat gattgtggtg catgcggaaa agccttcagt
      961 gagaagtttc atcttgttgt acatcagaga actcatactg gggagaaacc ttatgattgt
     1021 tctgaatgtg gaaaagcctt ctctcagaaa tcgtccctta ttatacatca gagagttcac
     1081 actggggaaa aaccctatga atgtagtgaa tgcgggaaag ccttctccca gaaatcaccc
     1141 ctcattatac atcagagaat acatactggg gaaaaaccct atgaatgtag agagtgtggg
     1201 aaggcctttt cccagaagtc acagctgatt atacaccaca gagctcatac tggagagaag
     1261 ccgtatgagt gtaccgaatg tgggaaagcc ttctgtgaga agtcccacct cattatacat
     1321 aaaagaattc acactggtga gaaaccctac aaatgtgctc aatgtgagga agccttcagc
     1381 aggaagacag aactcattac acatcagtta gttcatactg gggaaaaacc ttatgaatgt
     1441 actgaatgtg gaaagacatt ctcccgcaag tcacagctca tcatacatca gagaacacat
     1501 actggagaaa aaccctataa atgtagtgaa tgtggcaaag ccttctgcca gaagtcacat
     1561 ctcattggac atcagagaat tcacacagga gaaaaacctt atatatgtac tgaatgtggg
     1621 aaagccttct ctcagaagtc ccaccttccg ggacaccagc gaattcatac aggagagaaa
     1681 ccttacatat gtgctgaatg tggaaaggcc ttttctcaga agtcagacct tgttttacat
     1741 cagaggattc atactgggga aagaccctat caatgtgcta tatgtgggaa ggccttcatc
     1801 cagaagtcac aactaactgt acaccagaga attcacacag tggtaaaatc attgccaact
     1861 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1921 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1981 tcttgtggaa aggacgaaag aggatgtggt agccattttc c
//
