LOCUS       sequence-285924-        1592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3127
FEATURES             Location/Qualifiers
     source          1..1592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1424..1448)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1456..1497
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc agctgagaaa aatgcagacc
       61 gtcaaaaagg agcaggcgtc tcttgatgcc agtagcaatg tggacaagat gatggtcctt
      121 aattctgctt taacggaagt gtcagaagac tccacaacag gtgaggagct gcttctcagt
      181 gaaggaagtg tggggaagaa caaatcttct gcatgtcgga ggaaacggga attcattcct
      241 gatgaaaaga aagatgctat gtattgggaa aaaaggcgga aaaataatga agctgccaaa
      301 agatctcgtg agaagcgtcg actgaatgac ctggttttag agaacaaact aattgcactg
      361 ggagaagaaa acgccacttt aaaagctgag ctgctttcac taaaattaaa gtttggttta
      421 attagctcca cagcatatgc tcaagagatt cagaaactca gtaattctac agctgtgtac
      481 tttcaagatt accagacttc caaatccaat gtgagttcat ttgtggacga gcacgaaccc
      541 tcgatggtgt caagtagttg tatttctgtc attaaacact ctccacaaag ctcgctgtcc
      601 gatgtttcag aagtgtcctc agtagaacac acgcaggaga gctctgtgca gggaagctgc
      661 agaagtcctg aaaacaagtt ccagattatc aagcaagagc cgatggaatt agagagctac
      721 acaagggagc caagagatga ccgaggctct tacacagcgt ccatctatca aaactatatg
      781 gggaattctt tctctgggta ctcacactct cccccactac tgcaagtcaa ccgatcctcc
      841 agcaactccc cgagaacgtc ggaaactgat gatggtgtgg taggaaagtc atctgatgga
      901 gaagacgagc aacaggtccc caagggcccc atccattctc cagttgaact caagcatgtg
      961 catgcaactg tggttaaagt tccagaagtg aattcctctg ccttgccaca caagctccgg
     1021 atcaaagcca aagccatgca gatcaaagta gaagcctttg ataatgaatt tgaggccacg
     1081 caaaaacttt cctcacctat tgacatgaca tctaaaagac atttcgaact cgaaaagcat
     1141 agtgccccaa gtatggtaca ttcttctctt actcctttct cagtgcaagt gactaacatt
     1201 caagattggt ctctcaaatc ggagcactgg catcaaaaag aactgagtgg caaaactcag
     1261 aatagtttca aaactggagt tgttgaaatg aaagacagtg gctacaaagt ttctgaccca
     1321 gagaacttgt atttgaagca ggggatagca aacttatctg cagaggttgt ctcactcaag
     1381 agacttatag ccacacaacc aatctctgct tcagactctg ggtacccaac tttcttgtac
     1441 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1501 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1561 aaggacgagc cagcgaggag gccttgcgta ta
//
