LOCUS       sequence-285927-        1700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3128
FEATURES             Location/Qualifiers
     source          1..1700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1532..1556)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1564..1605
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga accagtccat cccagtggct
       61 cccaccccac cccgccgcgt gcggctgaag ccctggctgg tggcccaggt gaacagctgc
      121 cagtacccag ggcttcaatg ggtcaacggg gaaaagaaat tattctgcat cccctggagg
      181 catgccacaa ggcatggtcc cagccaggac ggagataaca ccatcttcaa ggcctgggcc
      241 aaggagacag ggaaatacac cgaaggcgtg gatgaagccg atccggccaa gtggaaggcc
      301 aacctgcgct gtgcccttaa caagagccgg gacttccgcc tcatctacga cgggccccgg
      361 gacatgccac ctcagcccta caagatctac gaggtctgct ccaatggccc tgctcccaca
      421 gactcccagc cccctgagga ttactctttt ggtgcaggag aggaggagga agaagaggaa
      481 gagctgcaga ggatgttgcc aagcctgagc ctcacagagg atgtcaagtg gccgcccact
      541 ctgcagccgc ccactctgcg gccgcctact ctgcagccgc ccactctgca gccgcccgtg
      601 gtgctgggtc cccctgctcc agaccccagc cccctggctc ctccccctgg caaccctgct
      661 ggcttcaggg agcttctctc tgaggtcctg gagcctgggc ccctgcctgc cagcctgccc
      721 cctgcaggcg aacagctcct gccagacctg ctgatcagcc cccacatgct gcctctgacc
      781 gacctggaga tcaagtttca gtaccggggg cggccacccc gggccctcac catcagcaac
      841 ccccatggct gccggctctt ctacagccag ctggaggcca cccaggagca ggtggaactc
      901 ttcggcccca taagcctgga gcaagtgcgc ttccccagcc ctgaggacat ccccagtgac
      961 aagcagcgct tctacacgaa ccagctgctg gatgtcctgg accgcgggct catcctccag
     1021 ctacagggcc aggaccttta tgccatccgc ctgtgtcagt gcaaggtgtt ctggagcggg
     1081 ccttgtgcct cagcccatga ctcatgcccc aaccccatcc agcgggaggt caagaccaag
     1141 cttttcagcc tggagcattt tctcaatgag ctcatcctgt tccaaaaggg ccagaccaac
     1201 accccaccac ccttcgagat cttcttctgc tttggggaag aatggcctga ccgcaaaccc
     1261 cgagagaaga agctcattac tgtacaggtg gtgcctgtag cagctcgact gctgctggag
     1321 atgttctcag gggagctatc ttggtcagct gatagtatcc ggctacagat ctcaaaccca
     1381 gacctcaaag accgcatggt ggagcaattc aaggagctcc atcacatctg gcagtcccag
     1441 cagcggttgc agcctgtggc ccaggcccct cctggagcag gccttggtgt tggccagggg
     1501 ccctggccta tgcacccagc tggcatgcaa tacccaactt tcttgtacaa agtggttgat
     1561 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1621 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaacc
     1681 tgcagtactt aagacttagg
//
