LOCUS       sequence-285930-        1487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3129
FEATURES             Location/Qualifiers
     source          1..1487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1319..1343)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1351..1392
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgttcaggac caaacgatct
       61 gcgctcgtcc ggcgtctctg gaggagccgt gcgcccggcg gcgaggacga ggaggagggc
      121 gcagggggag gtggaggagg aggcgagctg cggggagaag gggcgacgga cagccgagcg
      181 catggggccg gtggcggcgg cccgggcagg gctggatgct gcctgggcaa ggcggtgcga
      241 ggtgccaaag gtcaccacca tccccacccg ccagccgcgg gcgccggcgc ggccgggggc
      301 gccgaggcgg atctgaaggc gctcacgcac tcggtgctca agaaactgaa ggagcggcag
      361 ctggagctgc tgctccaggc cgtggagtcc cgcggcggga cgcgcaccgc gtgcctcctg
      421 ctgcccggcc gcctggactg caggctgggc ccgggggcgc ccgccggcgc gcagcctgcg
      481 cagccgccct cgtcctactc gctccccctc ctgctgtgca aagtgttcag gtggccggat
      541 ctcaggcatt cctcggaagt caagaggctg tgttgctgtg aatcttacgg gaagatcaac
      601 cccgagctgg tgtgctgcaa cccccatcac cttagccgac tctgcgaact agagtctccc
      661 ccccctcctt actccagata cccgatggat tttctcaaac caactgcaga ctgtccagat
      721 gctgtgcctt cctccgctga aacaggggga acgaattatc tggcccctgg ggggctttca
      781 gattcccaac ttcttctgga gcctggggat cggtcacact ggtgcgtggt ggcatactgg
      841 gaggagaaga cgagagtggg gaggctctac tgtgtccagg agccctctct ggatatcttc
      901 tatgatctac ctcaggggaa tggcttttgc ctcggacagc tcaattcgga caacaagagt
      961 cagctggtgc agaaggtgcg gagcaaaatc ggctgcggca tccagctgac gcgggaggtg
     1021 gatggtgtgt gggtgtacaa ccgcagcagt taccccatct tcatcaagtc cgccacactg
     1081 gacaacccgg actccaggac gctgttggta cacaaggtgt tccccggttt ctccatcaag
     1141 gctttcgact acgagaaggc gtacagcctg cagcggccca atgaccacga gtttatgcag
     1201 cagccgtgga cgggctttac cgtgcagatc agctttgtga agggctgggg ccagtgctac
     1261 acccgccagt tcatcagcag ctgcccgtgc tggctagagg tcatcttcaa cagccggttg
     1321 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     1381 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1441 tatatatctt gtggaaagga cgatcagatt ctgttacaga agtaaaa
//
