LOCUS       sequence-285936-        1694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3131
FEATURES             Location/Qualifiers
     source          1..1694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1526..1550)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1558..1599
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtcttctaa ttcagatact
       61 ggggatttac aagagtcttt aaagcacgga cttacaccta ttgtgtctca atttaaaatg
      121 gtgaattact cctatgatga agatctggaa gagctttgtc ccgtgtgtgg agataaagtg
      181 tctgggtacc attatgggct cctcacctgt gaaagctgca agggattttt taagcgaaca
      241 gtccaaaata ataaaaggta cacatgtata gaaaaccaga actgccaaat tgacaaaaca
      301 cagagaaagc gttgtcctta ctgtcgtttt caaaaatgtc taagtgttgg aatgaagcta
      361 gaagctgtaa gggccgaccg aatgcgtgga ggaaggaata agtttgggcc aatgtacaag
      421 agagacaggg ccctgaagca acagaaaaaa gccctcatcc gagccaatgg acttaagcta
      481 gaagccatgt ctcaggtgat ccaagctatg ccctctgacc tgaccatttc ctctgcaatt
      541 caaaacatcc actctgcctc caaaggccta cctctgaacc atgctgcctt gcctcctaca
      601 gactatgaca gaagtccctt tgtaacatcc cccattagca tgacaatgcc ccctcacggc
      661 agcctgcaag gttaccaaac atatggccac tttcctagcc gggccatcaa gtctgagtac
      721 ccagacccct ataccagctc acccgagtcc ataatgggct attcatatat ggatagttac
      781 cagacgagct ctccagcaag catcccacat ctgatactgg aacttttgaa gtgtgagcca
      841 gatgagcctc aagtccaggc taaaatcatg gcctatttgc agcaagagca ggctaaccga
      901 agcaagcacg aaaagctgag cacctttggg cttatgtgca aaatggcaga tcaaactctc
      961 ttctccattg tcgagtgggc caggagtagt atcttcttca gagaacttaa ggttgatgac
     1021 caaatgaagc tgcttcagaa ctgctggagt gagctcttaa tcctcgacca catttaccga
     1081 caagtggtac atggaaagga aggatccatc ttcctggtta ctgggcaaca agtggactat
     1141 tccataatag catcacaagc cggagccacc ctcaacaacc tcatgagtca tgcacaggag
     1201 ttagtggcaa aacttcgttc tctccagttt gatcaacgag agttcgtatg tctgaaattc
     1261 ttggtgctct ttagtttaga tgtcaaaaac cttgaaaact tccagctggt agaaggtgtc
     1321 caggaacaag tcaatgccgc cctgctggac tacacaatgt gtaactaccc gcagcagaca
     1381 gagaaatttg gacagctact tcttcgacta cccgaaatcc gggccatcag tatgcaggct
     1441 gaagaatacc tctactacaa gcacctgaac ggggatgtgc cctataataa ccttctcatt
     1501 gaaatgttgc atgccaaaag agcattgcca actttcttgt acaaagtggt tgatatcggt
     1561 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1621 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tccagctcac
     1681 agaaaagcgc cccc
//
