LOCUS       sequence-285939-        1802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3132
FEATURES             Location/Qualifiers
     source          1..1802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1634..1658)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1666..1707
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tggatataaa aaactcacca
       61 tctagcctta attctccttc ctcctacaac tgcagtcaat ccatcttacc cctggagcac
      121 ggctccatat acataccttc ctcctatgta gacagccacc atgaatatcc agccatgaca
      181 ttctatagcc ctgctgtgat gaattacagc attcccagca atgtcactaa cttggaaggt
      241 gggcctggtc ggcagaccac aagcccaaat gtgttgtggc caacacctgg gcacctttct
      301 cctttagtgg tccatcgcca gttatcacat ctgtatgcgg aacctcaaaa gagtccctgg
      361 tgtgaagcaa gatcgctaga acacacctta cctgtaaaca gagagacact gaaaaggaag
      421 gttagtggga accgttgcgc cagccctgtt actggtccag gttcaaagag ggatgctcac
      481 ttctgcgctg tctgcagcga ttacgcatcg ggatatcact atggagtctg gtcgtgtgaa
      541 ggatgtaagg ccttttttaa aagaagcatt caaggacata atgattatat ttgtccagct
      601 acaaatcagt gtacaatcga taaaaaccgg cgcaagagct gccaggcctg ccgacttcgg
      661 aagtgttacg aagtgggaat ggtgaagtgt ggctcccgga gagagagatg tgggtaccgc
      721 cttgtgcgga gacagagaag tgccgacgag cagctgcact gtgccggcaa ggccaagaga
      781 agtggcggcc acgcgccccg agtgcgggag ctgctgctgg acgccctgag ccccgagcag
      841 ctagtgctca ccctcctgga ggctgagccg ccccatgtgc tgatcagccg ccccagtgcg
      901 cccttcaccg aggcctccat gatgatgtcc ctgaccaagt tggccgacaa ggagttggta
      961 cacatgatca gctgggccaa gaagattccc ggctttgtgg agctcagcct gttcgaccaa
     1021 gtgcggctct tggagagctg ttggatggag gtgttaatga tggggctgat gtggcgctca
     1081 attgaccacc ccggcaagct catctttgct ccagatcttg ttctggacag ggatgagggg
     1141 aaatgcgtag aaggaattct ggaaatcttt gacatgctcc tggcaactac ttcaaggttt
     1201 cgagagttaa aactccaaca caaagaatat ctctgtgtca aggccatgat cctgctcaat
     1261 tccagtatgt accctctggt cacagcgacc caggatgctg acagcagccg gaagctggct
     1321 cacttgctga acgccgtgac cgatgctttg gtttgggtga ttgccaagag cggcatctcc
     1381 tcccagcagc aatccatgcg cctggctaac ctcctgatgc tcctgtccca cgtcaggcat
     1441 gcgagtaaca agggcatgga acatctgctc aacatgaagt gcaaaaatgt ggtcccagtg
     1501 tatgacctgc tgctggagat gctgaatgcc cacgtgcttc gcgggtgcaa gtcctccatc
     1561 acggggtccg agtgcagccc ggcagaggac agtaaaagca aagagggctc ccagaaccca
     1621 cagtctcagt gagacccagc tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1681 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1741 tcgatttctt ggctttatat atcttgtgga aaggacgata tgatcccccg ggcgtattta
     1801 gc
//
