LOCUS       sequence-285947-       15889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15889)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3135
FEATURES             Location/Qualifiers
     source          1..15889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4118..4142
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5880..5889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(11562..11586)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11594..11635
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    11746..12334
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11799..11819)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12217..12228)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12406..12639
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12717..12838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12754..12773)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12808..12827
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12878..13013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13033..13052)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13034..13052)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13062..13079)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13062..13078)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13071..13093)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13220..13675
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13307..13326)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13517..13538
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13701..13805
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13806..13874,13875..14666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14024..14043)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14837..15425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15326..15345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15579..15596
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15713..15734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15749..15754,15755..15772,15773..15779)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15787..15803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15792..15814
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15811..15827
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15811..15827
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15846..15866
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15848..15866
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcatcga ttgatcaaca agtttgtaca aaaaagcagg
     4141 caccatgccc gccctgcgcc ccgctctgct gtgggcgctg ctggcgctct ggctgtgctg
     4201 cgcggccccc gcgcatgcat tgcagtgtcg agatggctat gaaccctgtg taaatgaagg
     4261 aatgtgtgtt acctaccaca atggcacagg atactgcaaa tgtccagaag gcttcttggg
     4321 ggaatattgt caacatcgag acccctgtga gaagaaccgc tgccagaatg gtgggacttg
     4381 tgtggcccag gccatgctgg ggaaagccac gtgccgatgt gcctcagggt ttacaggaga
     4441 ggactgccag tactcaacat ctcatccatg ctttgtgtct cgaccctgcc tgaatggcgg
     4501 cacatgccat atgctcagcc gggataccta tgagtgcacc tgtcaagtcg ggtttacagg
     4561 taaggagtgc caatggacgg atgcctgcct gtctcatccc tgtgcaaatg gaagtacctg
     4621 taccactgtg gccaaccagt tctcctgcaa atgcctcaca ggcttcacag ggcagaaatg
     4681 tgagactgat gtcaatgagt gtgacattcc aggacactgc cagcatggtg gcacctgcct
     4741 caacctgcct ggttcctacc agtgccagtg ccctcagggc ttcacaggcc agtactgtga
     4801 cagcctgtat gtgccctgtg caccctcacc ttgtgtcaat ggaggcacct gtcggcagac
     4861 tggtgacttc acttttgagt gcaactgcct tccaggtttt gaagggagca cctgtgagag
     4921 gaatattgat gactgcccta accacaggtg tcagaatgga ggggtttgtg tggatggggt
     4981 caacacttac aactgccgct gtcccccaca atggacagga cagttctgca cagaggatgt
     5041 ggatgaatgc ctgctgcagc ccaatgcctg tcaaaatggg ggcacctgtg ccaaccgcaa
     5101 tggaggctat ggctgtgtat gtgtcaacgg ctggagtgga gatgactgca gtgagaacat
     5161 tgatgattgt gccttcgcct cctgtactcc aggctccacc tgcatcgacc gtgtggcctc
     5221 cttctcttgc atgtgcccag aggggaaggc aggtctcctg tgtcatctgg atgatgcatg
     5281 catcagcaat ccttgccaca agggggcact gtgtgacacc aaccccctaa atgggcaata
     5341 tatttgcacc tgcccacaag gctacaaagg ggctgactgc acagaagatg tggatgaatg
     5401 tgccatggcc aatagcaatc cttgtgagca tgcaggaaaa tgtgtgaaca cggatggcgc
     5461 cttccactgt gagtgtctga agggttatgc aggacctcgt tgtgagatgg acatcaatga
     5521 gtgccattca gacccctgcc agaatgatgc tacctgtctg gataagattg gaggcttcac
     5581 atgtctgtgc atgccaggtt tcaaaggtgt gcattgtgaa ttagaaataa atgaatgtca
     5641 gagcaaccct tgtgtgaaca atgggcagtg tgtggataaa gtcaatcgtt tccagtgcct
     5701 gtgtcctcct ggtttcactg ggccagtttg ccagattgat attgatgact gttccagtac
     5761 tccgtgtctg aatggggcaa agtgtatcga tcacccgaat ggctatgaat gccagtgtgc
     5821 cacaggtttc actggtgtgt tgtgtgagga gaacattgac aactgtgacc ccgatccttg
     5881 ccaccatggt cagtgtcagg atggtattga ttcctacacc tgcatctgca atcccgggta
     5941 catgggcgcc atctgcagtg accagattga tgaatgttac agcagccctt gcctgaacga
     6001 tggtcgctgc attgacctgg tcaatggcta ccagtgcaac tgccagccag gcacgtcagg
     6061 ggttaattgt gaaattaatt ttgatgactg tgcaagtaac ccttgtatcc atggaatctg
     6121 tatggatggc attaatcgct acagttgtgt ctgctcacca ggattcacag ggcagagatg
     6181 taacattgac attgatgagt gtgcctccaa tccctgtcgc aagggtgcaa catgtatcaa
     6241 cggtgtgaat ggtttccgct gtatatgccc cgagggaccc catcacccca gctgctactc
     6301 acaggtgaac gaatgcctga gcaatccctg catccatgga aactgtactg gaggtctcag
     6361 tggatataag tgtctctgtg atgcaggctg ggttggcatc aactgtgaag tggacaaaaa
     6421 tgaatgcctt tcgaatccat gccagaatgg aggaacttgt gacaatctgg tgaatggata
     6481 caggtgtact tgcaagaagg gctttaaagg ctataactgc caggtgaata ttgatgaatg
     6541 tgcctcaaat ccatgcctga accaaggaac ctgctttgat gacataagtg gctacacttg
     6601 ccactgtgtg ctgccataca caggcaagaa ttgtcagaca gtattggctc cctgttcccc
     6661 aaacccttgt gagaatgctg ctgtttgcaa agagtcacca aattttgaga gttatacttg
     6721 cttgtgtgct cctggctggc aaggtcagcg gtgtaccatt gacattgacg agtgtatctc
     6781 caagccctgc atgaaccatg gtctctgcca taacacccag ggcagctaca tgtgtgaatg
     6841 tccaccaggc ttcagtggta tggactgtga ggaggacatt gatgactgcc ttgccaatcc
     6901 ttgccagaat ggaggttcct gtatggatgg agtgaatact ttctcctgcc tctgccttcc
     6961 gggtttcact ggggataagt gccagacaga catgaatgag tgtctgagtg aaccctgtaa
     7021 gaatggaggg acctgctctg actacgtcaa cagttacact tgcaagtgcc aggcaggatt
     7081 tgatggagtc cattgtgaga acaacatcaa tgagtgcact gagagctcct gtttcaatgg
     7141 tggcacatgt gttgatggga ttaactcctt ctcttgcttg tgccctgtgg gtttcactgg
     7201 atccttctgc ctccatgaga tcaatgaatg cagctctcat ccatgcctga atgagggaac
     7261 gtgtgttgat ggcctgggta cctaccgctg cagctgcccc ctgggctaca ctgggaaaaa
     7321 ctgtcagacc ctggtgaatc tctgcagtcg gtctccatgt aaaaacaaag gtacttgcgt
     7381 tcagaaaaaa gcagagtccc agtgcctatg tccatctgga tgggctggtg cctattgtga
     7441 cgtgcccaat gtctcttgtg acatagcagc ctccaggaga ggtgtgcttg ttgaacactt
     7501 gtgccagcac tcaggtgtct gcatcaatgc tggcaacacg cattactgtc agtgccccct
     7561 gggctatact gggagctact gtgaggagca actcgatgag tgtgcgtcca acccctgcca
     7621 gcacggggca acatgcagtg acttcattgg tggatacaga tgcgagtgtg tcccaggcta
     7681 tcagggtgtc aactgtgagt atgaagtgga tgagtgccag aatcagccct gccagaatgg
     7741 aggcacctgt attgaccttg tgaaccattt caagtgctct tgcccaccag gcactcgggg
     7801 cctactctgt gaagagaaca ttgatgactg tgcccggggt ccccattgcc ttaatggtgg
     7861 tcagtgcatg gataggattg gaggctacag ttgtcgctgc ttgcctggct ttgctgggga
     7921 gcgttgtgag ggagacatca acgagtgcct ctccaacccc tgcagctctg agggcagcct
     7981 ggactgtata cagctcacca atgactacct gtgtgtttgc cgtagtgcct ttactggccg
     8041 gcactgtgaa accttcgtcg atgtgtgtcc ccagatgccc tgcctgaatg gagggacttg
     8101 tgctgtggcc agtaacatgc ctgatggttt catttgccgt tgtcccccgg gattttccgg
     8161 ggcaaggtgc cagagcagct gtggacaagt gaaatgtagg aagggggagc agtgtgtgca
     8221 caccgcctct ggaccccgct gcttctgccc cagtccccgg gactgcgagt caggctgtgc
     8281 cagtagcccc tgccagcacg ggggcagctg ccaccctcag cgccagcctc cttattactc
     8341 ctgccagtgt gccccaccat tctcgggtag ccgctgtgaa ctctacacgg caccccccag
     8401 cacccctcct gccacctgtc tgagccagta ttgtgccgac aaagctcggg atggcgtctg
     8461 tgatgaggcc tgcaacagcc atgcctgcca gtgggatggg ggtgactgtt ctctcaccat
     8521 ggagaacccc tgggccaact gctcctcccc acttccctgc tgggattata tcaacaacca
     8581 gtgtgatgag ctgtgcaaca cggtcgagtg cctgtttgac aactttgaat gccaggggaa
     8641 cagcaagaca tgcaagtatg acaaatactg tgcagaccac ttcaaagaca accactgtga
     8701 ccaggggtgc aacagtgagg agtgtggttg ggatgggctg gactgtgctg ctgaccaacc
     8761 tgagaacctg gcagaaggta ccctggttat tgtggtattg atgccacctg aacaactgct
     8821 ccaggatgct cgcagcttct tgcgggcact gggtaccctg ctccacacca acctgcgcat
     8881 taagcgggac tcccaggggg aactcatggt gtacccctat tatggtgaga agtcagctgc
     8941 tatgaagaaa cagaggatga cacgcagatc ccttcctggt gaacaagaac aggaggtggc
     9001 tggctctaaa gtctttctgg aaattgacaa ccgccagtgt gttcaagact cagaccactg
     9061 cttcaagaac acggatgcag cagcagctct cctggcctct cacgccatac aggggaccct
     9121 gtcataccct cttgtgtctg tcgtcagtga atccctgact ccagaacgca ctcagctcct
     9181 ctatctcctt gctgttgctg ttgtcatcat tctgtttatt attctgctgg gggtaatcat
     9241 ggcaaaacga aagcgtaagc atggctctct ctggctgcct gaaggtttca ctcttcgccg
     9301 agatgcaagc aatcacaagc gtcgtgagcc agtgggacag gatgctgtgg ggctgaaaaa
     9361 tctctcagtg caagtctcag aagctaacct aattggtact ggaacaagtg aacactgggt
     9421 cgatgatgaa gggccccagc caaagaaagt aaaggctgaa gatgaggcct tactctcaga
     9481 agaagatgac cccattgatc gacggccatg gacacagcag caccttgaag ctgcagacat
     9541 ccgtaggaca ccatcgctgg ctctcacccc tcctcaggca gagcaggagg tggatgtgtt
     9601 agatgtgaat gtccgtggcc cagatggctg caccccattg atgttggctt ctctccgagg
     9661 aggcagctca gatttgagtg atgaagatga agatgcagag gactcttctg ctaacatcat
     9721 cacagacttg gtctaccagg gtgccagcct ccaggcccag acagaccgga ctggtgagat
     9781 ggccctgcac cttgcagccc gctactcacg ggctgatgct gccaagcgtc tcctggatgc
     9841 aggtgcagat gccaatgccc aggacaacat gggccgctgt ccactccatg ctgcagtggc
     9901 agctgatgcc caaggtgtct tccagattct gattcgcaac cgagtaactg atctagatgc
     9961 caggatgaat gatggtacta cacccctgat cctggctgcc cgcctggctg tggagggaat
    10021 ggtggcagaa ctgatcaact gccaagcgga tgtgaatgca gtggatgacc atggaaaatc
    10081 tgctcttcac tgggcagctg ctgtcaataa tgtggaggca actcttttgt tgttgaaaaa
    10141 tggggccaac cgagacatgc aggacaacaa ggaagagaca cctctgtttc ttgctgcccg
    10201 ggaggggagc tatgaagcag ccaagatcct gttagaccat tttgccaatc gagacatcac
    10261 agaccatatg gatcgtcttc cccgggatgt ggctcgggat cgcatgcacc atgacattgt
    10321 gcgccttctg gatgaataca atgtgacccc aagccctcca ggcaccgtgt tgacttctgc
    10381 tctctcacct gtcatctgtg ggcccaacag atctttcctc agcctgaagc acaccccaat
    10441 gggcaagaag tctagacggc ccagtgccaa gagtaccatg cctactagcc tccctaacct
    10501 tgccaaggag gcaaaggatg ccaagggtag taggaggaag aagtctctga gtgagaaggt
    10561 ccaactgtct gagagttcag taactttatc ccctgttgat tccctagaat ctcctcacac
    10621 gtatgtttcc gacaccacat cctctccaat gattacatcc cctgggatct tacaggcctc
    10681 acccaaccct atgttggcca ctgccgcccc tcctgcccca gtccatgccc agcatgcact
    10741 atctttttct aaccttcatg aaatgcagcc tttggcacat ggggccagca ctgtgcttcc
    10801 ctcagtgagc cagttgctat cccaccacca cattgtgtct ccaggcagtg gcagtgctgg
    10861 aagcttgagt aggctccatc cagtcccagt cccagcagat tggatgaacc gcatggaggt
    10921 gaatgagacc cagtacaatg agatgtttgg tatggtcctg gctccagctg agggcaccca
    10981 tcctggcata gctccccaga gcaggccacc tgaagggaag cacataacca cccctcggga
    11041 gcccttgccc cccattgtga ctttccagct catccctaaa ggcagtattg cccaaccagc
    11101 gggggctccc cagcctcagt ccacctgccc tccagctgtt gcgggccccc tgcccaccat
    11161 gtaccagatt ccagaaatgg cccgtttgcc cagtgtggct ttccccactg ccatgatgcc
    11221 ccagcaggac gggcaggtag ctcagaccat tctcccagcc tatcatcctt tcccagcctc
    11281 tgtgggcaag taccccacac ccccttcaca gcacagttat gcttcctcaa atgctgctga
    11341 gcgaacaccc agtcacagtg gtcacctcca gggtgagcat ccctacctga caccatcccc
    11401 agagtctcct gaccagtggt caagttcatc accccactct gcttctgact ggtcagatgt
    11461 gaccaccagc cctacccctg ggggtgctgg aggaggtcag cggggacctg ggacacacat
    11521 gtctgagcca ccacacaaca acatgcaggt ttatgcgtga gacccagctt tcttgtacaa
    11581 agtggttgat atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta
    11641 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
    11701 ggacgagttc tataacaggg caccaccctg acgcgttaag tcgacaatca acctctggat
    11761 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
    11821 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
    11881 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
    11941 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
    12001 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
    12061 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
    12121 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
    12181 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
    12241 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
    12301 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
    12361 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
    12421 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
    12481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    12541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    12601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
    12661 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
    12721 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    12781 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    12841 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    12901 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    12961 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
    13021 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
    13081 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    13141 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    13201 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    13261 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    13321 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    13381 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    13441 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    13501 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    13561 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    13621 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
    13681 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    13741 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    13801 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    13861 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    13921 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    13981 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    14041 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    14101 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    14161 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    14221 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    14281 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    14341 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    14401 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    14461 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    14521 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    14581 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    14641 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    14701 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    14761 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    14821 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    14881 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    14941 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    15001 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    15061 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    15121 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    15181 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    15241 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    15301 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    15361 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    15421 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    15481 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    15541 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    15601 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    15661 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    15721 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    15781 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    15841 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
