LOCUS       sequence-285948-        7625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3135
FEATURES             Location/Qualifiers
     source          1..7625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1775..1784
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(7457..7481)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7489..7530
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tgcccgccct gcgccccgct
       61 ctgctgtggg cgctgctggc gctctggctg tgctgcgcgg cccccgcgca tgcattgcag
      121 tgtcgagatg gctatgaacc ctgtgtaaat gaaggaatgt gtgttaccta ccacaatggc
      181 acaggatact gcaaatgtcc agaaggcttc ttgggggaat attgtcaaca tcgagacccc
      241 tgtgagaaga accgctgcca gaatggtggg acttgtgtgg cccaggccat gctggggaaa
      301 gccacgtgcc gatgtgcctc agggtttaca ggagaggact gccagtactc aacatctcat
      361 ccatgctttg tgtctcgacc ctgcctgaat ggcggcacat gccatatgct cagccgggat
      421 acctatgagt gcacctgtca agtcgggttt acaggtaagg agtgccaatg gacggatgcc
      481 tgcctgtctc atccctgtgc aaatggaagt acctgtacca ctgtggccaa ccagttctcc
      541 tgcaaatgcc tcacaggctt cacagggcag aaatgtgaga ctgatgtcaa tgagtgtgac
      601 attccaggac actgccagca tggtggcacc tgcctcaacc tgcctggttc ctaccagtgc
      661 cagtgccctc agggcttcac aggccagtac tgtgacagcc tgtatgtgcc ctgtgcaccc
      721 tcaccttgtg tcaatggagg cacctgtcgg cagactggtg acttcacttt tgagtgcaac
      781 tgccttccag gttttgaagg gagcacctgt gagaggaata ttgatgactg ccctaaccac
      841 aggtgtcaga atggaggggt ttgtgtggat ggggtcaaca cttacaactg ccgctgtccc
      901 ccacaatgga caggacagtt ctgcacagag gatgtggatg aatgcctgct gcagcccaat
      961 gcctgtcaaa atgggggcac ctgtgccaac cgcaatggag gctatggctg tgtatgtgtc
     1021 aacggctgga gtggagatga ctgcagtgag aacattgatg attgtgcctt cgcctcctgt
     1081 actccaggct ccacctgcat cgaccgtgtg gcctccttct cttgcatgtg cccagagggg
     1141 aaggcaggtc tcctgtgtca tctggatgat gcatgcatca gcaatccttg ccacaagggg
     1201 gcactgtgtg acaccaaccc cctaaatggg caatatattt gcacctgccc acaaggctac
     1261 aaaggggctg actgcacaga agatgtggat gaatgtgcca tggccaatag caatccttgt
     1321 gagcatgcag gaaaatgtgt gaacacggat ggcgccttcc actgtgagtg tctgaagggt
     1381 tatgcaggac ctcgttgtga gatggacatc aatgagtgcc attcagaccc ctgccagaat
     1441 gatgctacct gtctggataa gattggaggc ttcacatgtc tgtgcatgcc aggtttcaaa
     1501 ggtgtgcatt gtgaattaga aataaatgaa tgtcagagca acccttgtgt gaacaatggg
     1561 cagtgtgtgg ataaagtcaa tcgtttccag tgcctgtgtc ctcctggttt cactgggcca
     1621 gtttgccaga ttgatattga tgactgttcc agtactccgt gtctgaatgg ggcaaagtgt
     1681 atcgatcacc cgaatggcta tgaatgccag tgtgccacag gtttcactgg tgtgttgtgt
     1741 gaggagaaca ttgacaactg tgaccccgat ccttgccacc atggtcagtg tcaggatggt
     1801 attgattcct acacctgcat ctgcaatccc gggtacatgg gcgccatctg cagtgaccag
     1861 attgatgaat gttacagcag cccttgcctg aacgatggtc gctgcattga cctggtcaat
     1921 ggctaccagt gcaactgcca gccaggcacg tcaggggtta attgtgaaat taattttgat
     1981 gactgtgcaa gtaacccttg tatccatgga atctgtatgg atggcattaa tcgctacagt
     2041 tgtgtctgct caccaggatt cacagggcag agatgtaaca ttgacattga tgagtgtgcc
     2101 tccaatccct gtcgcaaggg tgcaacatgt atcaacggtg tgaatggttt ccgctgtata
     2161 tgccccgagg gaccccatca ccccagctgc tactcacagg tgaacgaatg cctgagcaat
     2221 ccctgcatcc atggaaactg tactggaggt ctcagtggat ataagtgtct ctgtgatgca
     2281 ggctgggttg gcatcaactg tgaagtggac aaaaatgaat gcctttcgaa tccatgccag
     2341 aatggaggaa cttgtgacaa tctggtgaat ggatacaggt gtacttgcaa gaagggcttt
     2401 aaaggctata actgccaggt gaatattgat gaatgtgcct caaatccatg cctgaaccaa
     2461 ggaacctgct ttgatgacat aagtggctac acttgccact gtgtgctgcc atacacaggc
     2521 aagaattgtc agacagtatt ggctccctgt tccccaaacc cttgtgagaa tgctgctgtt
     2581 tgcaaagagt caccaaattt tgagagttat acttgcttgt gtgctcctgg ctggcaaggt
     2641 cagcggtgta ccattgacat tgacgagtgt atctccaagc cctgcatgaa ccatggtctc
     2701 tgccataaca cccagggcag ctacatgtgt gaatgtccac caggcttcag tggtatggac
     2761 tgtgaggagg acattgatga ctgccttgcc aatccttgcc agaatggagg ttcctgtatg
     2821 gatggagtga atactttctc ctgcctctgc cttccgggtt tcactgggga taagtgccag
     2881 acagacatga atgagtgtct gagtgaaccc tgtaagaatg gagggacctg ctctgactac
     2941 gtcaacagtt acacttgcaa gtgccaggca ggatttgatg gagtccattg tgagaacaac
     3001 atcaatgagt gcactgagag ctcctgtttc aatggtggca catgtgttga tgggattaac
     3061 tccttctctt gcttgtgccc tgtgggtttc actggatcct tctgcctcca tgagatcaat
     3121 gaatgcagct ctcatccatg cctgaatgag ggaacgtgtg ttgatggcct gggtacctac
     3181 cgctgcagct gccccctggg ctacactggg aaaaactgtc agaccctggt gaatctctgc
     3241 agtcggtctc catgtaaaaa caaaggtact tgcgttcaga aaaaagcaga gtcccagtgc
     3301 ctatgtccat ctggatgggc tggtgcctat tgtgacgtgc ccaatgtctc ttgtgacata
     3361 gcagcctcca ggagaggtgt gcttgttgaa cacttgtgcc agcactcagg tgtctgcatc
     3421 aatgctggca acacgcatta ctgtcagtgc cccctgggct atactgggag ctactgtgag
     3481 gagcaactcg atgagtgtgc gtccaacccc tgccagcacg gggcaacatg cagtgacttc
     3541 attggtggat acagatgcga gtgtgtccca ggctatcagg gtgtcaactg tgagtatgaa
     3601 gtggatgagt gccagaatca gccctgccag aatggaggca cctgtattga ccttgtgaac
     3661 catttcaagt gctcttgccc accaggcact cggggcctac tctgtgaaga gaacattgat
     3721 gactgtgccc ggggtcccca ttgccttaat ggtggtcagt gcatggatag gattggaggc
     3781 tacagttgtc gctgcttgcc tggctttgct ggggagcgtt gtgagggaga catcaacgag
     3841 tgcctctcca acccctgcag ctctgagggc agcctggact gtatacagct caccaatgac
     3901 tacctgtgtg tttgccgtag tgcctttact ggccggcact gtgaaacctt cgtcgatgtg
     3961 tgtccccaga tgccctgcct gaatggaggg acttgtgctg tggccagtaa catgcctgat
     4021 ggtttcattt gccgttgtcc cccgggattt tccggggcaa ggtgccagag cagctgtgga
     4081 caagtgaaat gtaggaaggg ggagcagtgt gtgcacaccg cctctggacc ccgctgcttc
     4141 tgccccagtc cccgggactg cgagtcaggc tgtgccagta gcccctgcca gcacgggggc
     4201 agctgccacc ctcagcgcca gcctccttat tactcctgcc agtgtgcccc accattctcg
     4261 ggtagccgct gtgaactcta cacggcaccc cccagcaccc ctcctgccac ctgtctgagc
     4321 cagtattgtg ccgacaaagc tcgggatggc gtctgtgatg aggcctgcaa cagccatgcc
     4381 tgccagtggg atgggggtga ctgttctctc accatggaga acccctgggc caactgctcc
     4441 tccccacttc cctgctggga ttatatcaac aaccagtgtg atgagctgtg caacacggtc
     4501 gagtgcctgt ttgacaactt tgaatgccag gggaacagca agacatgcaa gtatgacaaa
     4561 tactgtgcag accacttcaa agacaaccac tgtgaccagg ggtgcaacag tgaggagtgt
     4621 ggttgggatg ggctggactg tgctgctgac caacctgaga acctggcaga aggtaccctg
     4681 gttattgtgg tattgatgcc acctgaacaa ctgctccagg atgctcgcag cttcttgcgg
     4741 gcactgggta ccctgctcca caccaacctg cgcattaagc gggactccca gggggaactc
     4801 atggtgtacc cctattatgg tgagaagtca gctgctatga agaaacagag gatgacacgc
     4861 agatcccttc ctggtgaaca agaacaggag gtggctggct ctaaagtctt tctggaaatt
     4921 gacaaccgcc agtgtgttca agactcagac cactgcttca agaacacgga tgcagcagca
     4981 gctctcctgg cctctcacgc catacagggg accctgtcat accctcttgt gtctgtcgtc
     5041 agtgaatccc tgactccaga acgcactcag ctcctctatc tccttgctgt tgctgttgtc
     5101 atcattctgt ttattattct gctgggggta atcatggcaa aacgaaagcg taagcatggc
     5161 tctctctggc tgcctgaagg tttcactctt cgccgagatg caagcaatca caagcgtcgt
     5221 gagccagtgg gacaggatgc tgtggggctg aaaaatctct cagtgcaagt ctcagaagct
     5281 aacctaattg gtactggaac aagtgaacac tgggtcgatg atgaagggcc ccagccaaag
     5341 aaagtaaagg ctgaagatga ggccttactc tcagaagaag atgaccccat tgatcgacgg
     5401 ccatggacac agcagcacct tgaagctgca gacatccgta ggacaccatc gctggctctc
     5461 acccctcctc aggcagagca ggaggtggat gtgttagatg tgaatgtccg tggcccagat
     5521 ggctgcaccc cattgatgtt ggcttctctc cgaggaggca gctcagattt gagtgatgaa
     5581 gatgaagatg cagaggactc ttctgctaac atcatcacag acttggtcta ccagggtgcc
     5641 agcctccagg cccagacaga ccggactggt gagatggccc tgcaccttgc agcccgctac
     5701 tcacgggctg atgctgccaa gcgtctcctg gatgcaggtg cagatgccaa tgcccaggac
     5761 aacatgggcc gctgtccact ccatgctgca gtggcagctg atgcccaagg tgtcttccag
     5821 attctgattc gcaaccgagt aactgatcta gatgccagga tgaatgatgg tactacaccc
     5881 ctgatcctgg ctgcccgcct ggctgtggag ggaatggtgg cagaactgat caactgccaa
     5941 gcggatgtga atgcagtgga tgaccatgga aaatctgctc ttcactgggc agctgctgtc
     6001 aataatgtgg aggcaactct tttgttgttg aaaaatgggg ccaaccgaga catgcaggac
     6061 aacaaggaag agacacctct gtttcttgct gcccgggagg ggagctatga agcagccaag
     6121 atcctgttag accattttgc caatcgagac atcacagacc atatggatcg tcttccccgg
     6181 gatgtggctc gggatcgcat gcaccatgac attgtgcgcc ttctggatga atacaatgtg
     6241 accccaagcc ctccaggcac cgtgttgact tctgctctct cacctgtcat ctgtgggccc
     6301 aacagatctt tcctcagcct gaagcacacc ccaatgggca agaagtctag acggcccagt
     6361 gccaagagta ccatgcctac tagcctccct aaccttgcca aggaggcaaa ggatgccaag
     6421 ggtagtagga ggaagaagtc tctgagtgag aaggtccaac tgtctgagag ttcagtaact
     6481 ttatcccctg ttgattccct agaatctcct cacacgtatg tttccgacac cacatcctct
     6541 ccaatgatta catcccctgg gatcttacag gcctcaccca accctatgtt ggccactgcc
     6601 gcccctcctg ccccagtcca tgcccagcat gcactatctt tttctaacct tcatgaaatg
     6661 cagcctttgg cacatggggc cagcactgtg cttccctcag tgagccagtt gctatcccac
     6721 caccacattg tgtctccagg cagtggcagt gctggaagct tgagtaggct ccatccagtc
     6781 ccagtcccag cagattggat gaaccgcatg gaggtgaatg agacccagta caatgagatg
     6841 tttggtatgg tcctggctcc agctgagggc acccatcctg gcatagctcc ccagagcagg
     6901 ccacctgaag ggaagcacat aaccacccct cgggagccct tgccccccat tgtgactttc
     6961 cagctcatcc ctaaaggcag tattgcccaa ccagcggggg ctccccagcc tcagtccacc
     7021 tgccctccag ctgttgcggg ccccctgccc accatgtacc agattccaga aatggcccgt
     7081 ttgcccagtg tggctttccc cactgccatg atgccccagc aggacgggca ggtagctcag
     7141 accattctcc cagcctatca tcctttccca gcctctgtgg gcaagtaccc cacaccccct
     7201 tcacagcaca gttatgcttc ctcaaatgct gctgagcgaa cacccagtca cagtggtcac
     7261 ctccagggtg agcatcccta cctgacacca tccccagagt ctcctgacca gtggtcaagt
     7321 tcatcacccc actctgcttc tgactggtca gatgtgacca ccagccctac ccctgggggt
     7381 gctggaggag gtcagcgggg acctgggaca cacatgtctg agccaccaca caacaacatg
     7441 caggtttatg cgtgagaccc agctttcttg tacaaagtgg ttgatatcgg taagcctatc
     7501 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     7561 atttcgattt cttggcttta tatatcttgt ggaaaggacg agttctataa cagggcacca
     7621 ccctg
//
