LOCUS       sequence-285951-        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3136
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(950..974)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             982..1023
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga agcgagctca ccccgagtac
       61 agctcctcgg acagcgagct ggacgagacc atcgaggtgg agaaggagag tgcggacgag
      121 aatggaaact tgagttcggc tctaggttcc atgtccccaa ctacatcttc ccagattttg
      181 gccagaaaaa gacggagagg aataattgag aagcgccgac gagaccggat caataacagt
      241 ttgtctgagc tgagaaggct ggtacccagt gcttttgaga agcagggatc tgctaagcta
      301 gaaaaagccg agatcctgca gatgaccgtg gatcacctga aaatgctgca tacggcagga
      361 gggaaaggtt actttgacgc gcacgccctt gctatggact atcggagttt gggatttcgg
      421 gaatgcctgg cagaagttgc gcgttatctg agcatcattg aaggactaga tgcctctgac
      481 ccgcttcgag ttcgactggt ttcgcatctc aacaactacg cttcccagcg ggaagccgcg
      541 agcggcgccc acgcgggcct cggacacatt ccctggggga ccgtcttcgg acatcacccg
      601 cacatcgcgc acccgctgtt gctgccccag aacggccacg ggaacgcggg caccacggcc
      661 tcacccacgg aaccgcacca ccagggcagg ctgggctcgg cacatccgga ggcgcctgct
      721 ttgcgagcgc cccctagcgg cagcctcgga ccggtgctcc ctgtggtcac ctccgcctcc
      781 aaactgtcgc cgcctctgct ctcctcagtg gcctccctgt cggccttccc cttctctttc
      841 ggctccttcc acttactgtc tcccaatgca ctgagccctt cagcacccac gcaggctgca
      901 aaccttggca agccctatag accttggggg acggagatcg gagcttttta cccaactttc
      961 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1021 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1081 tgtggaaagg acgaaccccg tgcaggggat gctaggtg
//
