LOCUS       sequence-285954-        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3137
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(968..992)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1000..1041
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tgaagcgagc tcaccccgag
       61 tacagctcct cggacagcga gctggacgag accatcgagg tggagaagga gagtgcggac
      121 gagaatggaa acttgagttc ggctctaggt tccatgtccc caactacatc ttcccagatt
      181 ttggccagaa aaagacggag aggaataatt gagaagcgcc gacgagaccg gatcaataac
      241 agtttgtctg agctgagaag gctggtaccc agtgcttttg agaagcaggt aatggagcaa
      301 ggatctgcta agctagaaaa agccgagatc ctgcagatga ccgtggatca cctgaaaatg
      361 ctgcatacgg caggagggaa aggttacttt gacgcgcacg cccttgctat ggactatcgg
      421 agtttgggat ttcgggaatg cctggcagaa gttgcgcgtt atctgagcat cattgaagga
      481 ctagatgcct ctgacccgct tcgagttcga ctggtttcgc atctcaacaa ctacgcttcc
      541 cagcgggaag ccgcgagcgg cgcccacgcg ggcctcggac acattccctg ggggaccgtc
      601 ttcggacatc acccgcacat cgcgcacccg ctgttgctgc cccagaacgg ccacgggaac
      661 gcgggcacca cggcctcacc cacggaaccg caccaccagg gcaggctggg ctcggcacat
      721 ccggaggcgc ctgctttgcg agcgccccct agcggcagcc tcggaccggt gctccctgtg
      781 gtcacctccg cctccaaact gtcgccgcct ctgctctcct cagtggcctc cctgtcggcc
      841 ttccccttct ctttcggctc cttccactta ctgtctccca atgcactgag cccttcagca
      901 cccacgcagg ctgcaaacct tggcaagccc tatagacctt gggggacgga gatcggagct
      961 ttttaagacc cagctttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1021 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1081 tcttggcttt atatatcttg tggaaaggac gaccatctac gcctgccggg cgggcg
//
