LOCUS       sequence-285957-        1637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3138
FEATURES             Location/Qualifiers
     source          1..1637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1469..1493)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1501..1542
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga ccatggttga cacagagatg
       61 ccattctggc ccaccaactt tgggatcagc tccgtggatc tctccgtaat ggaagaccac
      121 tcccactcct ttgatatcaa gcccttcact actgttgact tctccagcat ttctactcca
      181 cattacgaag acattccatt cacaagaaca gatccagtgg ttgcagatta caagtatgac
      241 ctgaaacttc aagagtacca aagtgcaatc aaagtggagc ctgcatctcc accttattat
      301 tctgagaaga ctcagctcta caataagcct catgaagagc cttccaactc cctcatggca
      361 attgaatgtc gtgtctgtgg agataaagct tctggatttc actatggagt tcatgcttgt
      421 gaaggatgca agggtttctt ccggagaaca atcagattga agcttatcta tgacagatgt
      481 gatcttaact gtcggatcca caaaaaaagt agaaataaat gtcagtactg tcggtttcag
      541 aaatgccttg cagtggggat gtctcataat gccatcaggt ttgggcggat gccacaggcc
      601 gagaaggaga agctgttggc ggagatctcc agtgatatcg accagctgaa tccagagtcc
      661 gctgacctcc gggccctggc aaaacatttg tatgactcat acataaagtc cttcccgctg
      721 accaaagcaa aggcgagggc gatcttgaca ggaaagacaa cagacaaatc accattcgtt
      781 atctatgaca tgaattcctt aatgatggga gaagataaaa tcaagttcaa acacatcacc
      841 cccctgcagg agcagagcaa agaggtggcc atccgcatct ttcagggctg ccagtttcgc
      901 tccgtggagg ctgtgcagga gatcacagag tatgccaaaa gcattcctgg ttttgtaaat
      961 cttgacttga acgaccaagt aactctcctc aaatatggag tccacgagat catttacaca
     1021 atgctggcct ccttgatgaa taaagatggg gttctcatat ccgagggcca aggcttcatg
     1081 acaagggagt ttctaaagag cctgcgaaag ccttttggtg actttatgga gcccaagttt
     1141 gagtttgctg tgaagttcaa tgcactggaa ttagatgaca gcgacttggc aatatttatt
     1201 gctgtcatta ttctcagtgg agaccgccca ggtttgctga atgtgaagcc cattgaagac
     1261 attcaagaca acctgctaca agccctggag ctccagctga agctgaacca ccctgagtcc
     1321 tcacagctgt ttgccaagct gctccagaaa atgacagacc tcagacagat tgtcacggaa
     1381 cacgtgcagc tactgcaggt gatcaagaag acggagacag acatgagtct tcacccgctc
     1441 ctgcaggaga tctacaagga cttgtactac ccaactttct tgtacaaagt ggttgatatc
     1501 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1561 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgagtataac
     1621 gccggagatc cacgaca
//
