LOCUS       sequence-285960-        1727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3139
FEATURES             Location/Qualifiers
     source          1..1727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1559..1583)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1591..1632
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tgggtgaaac tctgggagat
       61 tctcctattg acccagaaag cgattccttc actgatacac tgtctgcaaa catatcacaa
      121 gaaatgacca tggttgacac agagatgcca ttctggccca ccaactttgg gatcagctcc
      181 gtggatctct ccgtaatgga agaccactcc cactcctttg atatcaagcc cttcactact
      241 gttgacttct ccagcatttc tactccacat tacgaagaca ttccattcac aagaacagat
      301 ccagtggttg cagattacaa gtatgacctg aaacttcaag agtaccaaag tgcaatcaaa
      361 gtggagcctg catctccacc ttattattct gagaagactc agctctacaa taagcctcat
      421 gaagagcctt ccaactccct catggcaatt gaatgtcgtg tctgtggaga taaagcttct
      481 ggatttcact atggagttca tgcttgtgaa ggatgcaagg gtttcttccg gagaacaatc
      541 agattgaagc ttatctatga cagatgtgat cttaactgtc ggatccacaa aaaaagtaga
      601 aataaatgtc agtactgtcg gtttcagaaa tgccttgcag tggggatgtc tcataatgcc
      661 atcaggtttg ggcggatgcc acaggccgag aaggagaagc tgttggcgga gatctccagt
      721 gatatcgacc agctgaatcc agagtccgct gacctccggg ccctggcaaa acatttgtat
      781 gactcataca taaagtcctt cccgctgacc aaagcaaagg cgagggcgat cttgacagga
      841 aagacaacag acaaatcacc attcgttatc tatgacatga attccttaat gatgggagaa
      901 gataaaatca agttcaaaca catcaccccc ctgcaggagc agagcaaaga ggtggccatc
      961 cgcatctttc agggctgcca gtttcgctcc gtggaggctg tgcaggagat cacagagtat
     1021 gccaaaagca ttcctggttt tgtaaatctt gacttgaacg accaagtaac tctcctcaaa
     1081 tatggagtcc acgagatcat ttacacaatg ctggcctcct tgatgaataa agatggggtt
     1141 ctcatatccg agggccaagg cttcatgaca agggagtttc taaagagcct gcgaaagcct
     1201 tttggtgact ttatggagcc caagtttgag tttgctgtga agttcaatgc actggaatta
     1261 gatgacagcg acttggcaat atttattgct gtcattattc tcagtggaga ccgcccaggt
     1321 ttgctgaatg tgaagcccat tgaagacatt caagacaacc tgctacaagc cctggagctc
     1381 cagctgaagc tgaaccaccc tgagtcctca cagctgtttg ccaagctgct ccagaaaatg
     1441 acagacctca gacagattgt cacggaacat gtgcagctac tgcaggtgat caagaagacg
     1501 gagacagaca tgagtcttca cccgctcctg caggagatct acaaggactt gtactagaac
     1561 ccagctttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     1621 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1681 tatatatctt gtggaaagga cgagcaatgg cgctctcaga cgtagtc
//
