LOCUS       sequence-285963-        1643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3140
FEATURES             Location/Qualifiers
     source          1..1643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1475..1499)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1507..1548
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggaactctt aacattcaag
       61 gatgtggcca tagaattctc tccagaagag tggaaatgtc tggacacttc ccagcagaat
      121 ttatatagag atgtgatgtt ggagaactac agaaacctgg tctccctggg tgttagtatc
      181 tctaacccag acctggtcac cagtctggag caaagaaaag agccctacaa tttgaagata
      241 catgaaacag cagccagacc cccagctgtg tgttctcatt tcacccaaaa cctttggaca
      301 gtgcagggca tagaagattc attccacaaa cttataccaa aaggacatga gaaacgtgga
      361 catgagaatt taagaaaaac ttgtaaaagt ataaatgagt gtaaggtgca gaaaggtggt
      421 tataatagaa ttaaccaatg cttattaact acccagaaaa aaacaattca atctaatata
      481 tgtgtcaaag tttttcataa attttcaaat tcaaacaaag ataagataag atatactgga
      541 gataaaacct ttaaatgtaa agaatgtggc aaatcatttc acgtgctctc acgcctaact
      601 caacacaaaa gaattcatac tggagagaac ccctacacat gtgaagaatg tggcaaagcc
      661 tttaattggt cctcaattct tactaaacat aagagaattc atgccagaga gaaattctac
      721 aagtgtgaag aatgtggtaa aggctttact cggtcctcac accttactaa acataagaga
      781 attcatactg gagagaaacc ctacatatgt gaaaaatgtg gtaaagcttt taaccaatcc
      841 tcaaccctta atttacataa gagaattcat tctgcacaaa aatactacaa atgtgaagaa
      901 tgtggtaaag cctttaagtg gtcctcatcc cttaatgaac ataagagaat tcatgctgga
      961 gagaaaccct tctcatgcga agaatgtggc aatgtcttta ccacatcctc agactttgct
     1021 aaacataaga gaattcatac aggagagaaa ccctacaaat gtgaagaatg tggaaaatcc
     1081 tttaataggt ccacaactct tacgacacat aagagaatcc atactggaga gaaaccctac
     1141 acatgtgaag aatgtggaaa agcctttaat tggtcctcaa cccttaatgt acacaagaga
     1201 attcactctg gaaaaaatcc ctacaaatgt gaagattgtg gcaaagcctt taaagtgttt
     1261 gcaaacctgc ataatcataa gaaaattcat actggagaga aaccctacat atgtaaacaa
     1321 tgtggcaaag cctttaaaca gtcctcacac ttgaataaac ataagaaaat tcacactgta
     1381 gataaaccct acaaatgtaa agaatgcggg aaagctttta agcagtactc caaccttcct
     1441 caacataaga gaactcatac tggaggaaaa tttttgccaa ctttcttgta caaagtggtt
     1501 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1561 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     1621 caaggcctcg tttgtgtgcg gag
//
