LOCUS       sequence-285966-        1238 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3141
FEATURES             Location/Qualifiers
     source          1..1238
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1070..1094)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1102..1143
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggaccccga gcactgcgcg
       61 cctttccgcg tggggcctgc acccggcccc tatgtggcct cgggggacga gcctccgggc
      121 ccgcagggaa cccccgccgc tgcgcctcac ctgcaccccg cgccgccccg cggcccgcgg
      181 ctgacccgct ttccggcctg cgggcccctg gagccctacc tcccagagcc ggccaagccg
      241 cccgccaagt acctgcagga cctcgggccc ggcccggccc tcaacggcgg ccacttctac
      301 gagggccccg cggaagctga ggagaagacc tccaaagctg ccagcttccc ccagctgccc
      361 ttggactgcc gagggggccc cagagacggg ccctctaact tgcaaggctc cccaggcccc
      421 tgcctggcca gcctgcatct tcctctttcc ccgggactcc ctgactccat ggagttggcc
      481 aagaacaaga gcaagaagcg tcgtaaccgc acgaccttca gcacattcca gctggaggag
      541 ctggagaagg tcttccagaa aacccactat cctgatgtgt atgcccggga gcagctggcc
      601 ctgcgcacag acctgactga ggcccgggta caggtctggt tccagaaccg cagagccaag
      661 tggcggaagc gcgagcgtta tgggaagatc caggaggggc ggaacccctt cacggctgcc
      721 tatgacatct ctgtgctgcc ccgtactgac agccaccctc agctgcagaa ctccctgtgg
      781 gccagtccag gatctgggag ccctggaggc ccctgccttg tgtctccaga gggcatcccc
      841 tccccatgca tgtctccata ttcccacccc catgggagtg tggctggctt catgggggtg
      901 ccagcccctt ctgcggctca ccctggcatc tactccatcc atggctttcc ccccaccctg
      961 gggggccaca gctttgagcc ttcctcagat ggtgactata agtctccaag cctcgtctcg
     1021 ctcagggtaa agcccaagga gccacccggc cttctgaact ggaccacgtt gccaactttc
     1081 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1141 acgtagtaat gaactagccc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1201 tgtggaaagg acgacaatgc gggaatgaag ttatcggt
//
