LOCUS       sequence-285969-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3142
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(893..917)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             925..966
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtacggaag ctgtcttttg
       61 gagaaagaag caggcatgta cccgggcact ctcatgagcc ctgggggcga cggcacagct
      121 gggacaggcg gcacaggggg cggtgggagt ccgatgccag cctccaattt cgctgcggca
      181 ccggctttct cgcactatat ggggtatcct catatgccca gcatggatcc tcactggccg
      241 tctctgggag tctggggctc accctacagt cccccgcgag aagactggag cgtgtatcct
      301 gggccgtcta gtacaatggg cacagtgccg gtgaacgacg tgacctctag ccccgccgct
      361 ttctgctcga ccgactacag caacttgggc cctgtgggcg gtggaactag cggcagcagc
      421 ctaccaggcc aggctggcgg gtcgcttgtc ccgacggacg caggcgccgc caaggccagt
      481 tcccccagca ggagccgcca cagcccctat gcatggatgc gcaagacggt gcaggtgacg
      541 gggaaaacca ggacaaaaga aaagtatcgt gtagtttaca ctgatcatca aagattggag
      601 ctggaaaagg aattccattg caatagatat atcaccatcc agagaaaatc agagctggca
      661 gttaacctgg gcctttccga gagacaggtg aaaatctggt ttcagaatcg cagagccaag
      721 gagagaaaga tgatcaaaaa gaaaatctcc cagtttgaga atagtggagg ctcggtgcaa
      781 agtgactctg actccatcag ccctggggaa ctacctaaca cttttttcac cacaccatct
      841 gctgttcgtg gatttcaacc tattgagata cagcaggtta tagtctccga attgccaact
      901 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
      961 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1021 tcttgtggaa aggacgatgt tgaatcggta acgatgacct g
//
