LOCUS       sequence-285972-        1493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3143
FEATURES             Location/Qualifiers
     source          1..1493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1325..1349)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1357..1398
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt caggcgtccg acctccgatc
       61 atgaacgggc ccctgcaccc gcggcccctg gtggcattgc tggatggccg ggactgcaca
      121 gtggagatgc ccatcctgaa ggacgtggcc actgtggcct tctgcgacgc gcagtccacg
      181 caggagatcc atgagaaggt cctgaacgag gctgtggggg ccctgatgta ccacaccatc
      241 actctcacca gggaggacct ggagaagttc aaagccctcc gcatcatcgt ccggattggc
      301 agtggttttg acaacatcga catcaagtcg gccggggatt taggcattgc cgtctgcaac
      361 gtgcccgcgg cgtctgtgga ggagacggcc gactcgacgc tgtgccacat cctgaacctg
      421 taccggcggg ccacctggct gcaccaggcg ctgcgggagg gcacacgagt ccagagcgtc
      481 gagcagatcc gcgaggtggc gtccggcgct gccaggatcc gcggggagac cttgggcatc
      541 atcggacttg gtcgcgtggg gcaggcagtg gcgctgcggg ccaaggcctt cggcttcaac
      601 gtgctcttct acgaccctta cttgtcggat ggcgtggagc gggcgctggg gctgcagcgt
      661 gtcagcaccc tgcaggacct gctcttccac agcgactgcg tgaccctgca ctgcggcctc
      721 aacgagcaca accaccacct catcaacgac ttcaccgtca agcagatgag acaaggggcc
      781 ttcctggtga acacagcccg gggtggcctg gtggatgaga aggcgctggc ccaggccctg
      841 aaggagggcc ggatccgcgg cgcggccctg gatgtgcacg agtcggaacc cttcagcttt
      901 agccagggcc ctctgaagga tgcacccaac ctcatctgca ccccccatgc tgcatggtac
      961 agcgagcagg catccatcga gatgcgagag gaggcggcac gggagatccg cagagccatc
     1021 acaggccgga tcccagacag cctgaagaac tgtgtcaaca aggaccatct gacagccgcc
     1081 acccactggg ccagcatgga ccccgccgtc gtgcaccctg agctcaatgg ggctgcctat
     1141 aggtaccctc cgggcgtggt gggcgtggcc cccactggca tcccagctgc tgtggaaggt
     1201 atcgtcccca gcgccatgtc cctgtcccac ggcctgcccc ctgtggccca cccgccccac
     1261 gccccttctc ctggccaaac cgtcaagccc gaggcggata gagaccacgc cagtgaccag
     1321 ttgtacccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1381 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1441 tggctttata tatcttgtgg aaaggacgac gcgtgttgct tgacggccta acc
//
