LOCUS       sequence-285975-        1733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3144
FEATURES             Location/Qualifiers
     source          1..1733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1565..1589)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1597..1638
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gctctggaga ccatcagttc
       61 aacctcgcag agatcctgtc acagaactac agtgttaggg gggagtgcga ggaggcctcg
      121 aggtgcccag acaagcccaa ggaggagctg gagaaggact tcatctccca gagcaacgac
      181 atgccctttg atgagctgct tgcgctctat ggctacgagg cgtcagaccc catttcagac
      241 cgggagagtg agggtggtga cgtggccccg aacctcccag acatgaccct ggacaaagaa
      301 caaatagcga aggatttgct ttcaggggaa gaagaggaag agacgcaatc atctgctgac
      361 gacctcaccc cgtccgtgac ctcccacgag gcctccgacc tcttccctaa ccggagtgga
      421 tctcgtttcc tggctgatga agacagagag cctggctctt ctgcctcctc cgacaccgag
      481 gaggactctc ttcctgccaa caaatgtaag aaggagatca tggtgggacc tcagttccaa
      541 gctgacctca gcaacctgca cttgaaccgg cactgtgaga agatctacga gaacgaagac
      601 cagctgctct gggaccccag cgtcctccct gagagggagg tggaggagtt cctgtacagg
      661 gcggtgaagc ggcgttggca cgagatggcc gggcctcagc tcccagaggg agaagccgtg
      721 aaagacagtg agcaggcgct gtacgagttg gtgaaatgca acttcaatgt ggaggaggcc
      781 ctgcgaaggc tgcggttcaa cgtgaaggtg atccgagatg ggctctgtgc ttggagtgaa
      841 gaggagtgca ggaactttga gcacggcttc cgtgtgcatg gaaagaactt tcacctgatc
      901 caggccaaca aggtgcgcac acggtcagtg ggcgagtgtg tcgagtacta ctacctgtgg
      961 aagaagtcgg agcgctacga ctacttcgcc cagcagacgc ggctgggccg gaggaagtac
     1021 gtcccgtccg gaaccacgga cgcagaccag gacctggatg gcagcgaccc cgatggcccc
     1081 ggccgtccgc gcccggagca agacaccctg actgggatgc gcacagatcc actgagcgtg
     1141 gatggcacgg ccggtggtct cgatgagccc ggagtggcct ctgatggact cccgtcctcg
     1201 gagccagggc cgtgttcctt ccagcagctg gatgagtccc ccgctgtacc cctgtcccat
     1261 cggcccccag ccctggccga cccagcctca taccagccag ctgtcactgc tccggagcca
     1321 gacgccagcc caaggctggc cgtggacttc gccctgccca aggagctgcc cctcatctcc
     1381 agccatgtgg acctcagcgg ggatccggag gagactgtgg ccccagcaca ggtggctttg
     1441 tcggtcaccg agtttggact catcggcatt ggggacgtga accccttcct ggccgcccac
     1501 cccacgtgcc cggcccccgg gctacactcg gagcccctgt cacactgtaa cgtgatgacc
     1561 tgctgcccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1621 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1681 tggctttata tatcttgtgg aaaggacgat ctacgtccgg ctgctcgtat cta
//
