LOCUS       sequence-285981-        1793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3146
FEATURES             Location/Qualifiers
     source          1..1793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1252..1623
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     protein_bind    complement(1625..1649)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1657..1698
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg atctgcccgt gggccccggc
       61 gcggcggggc ccagcaacgt cccggccttc ctgaccaagc tgtggaccct cgtgagcgac
      121 ccggacaccg acgcgctcat ctgctggagc ccgagcggga acagcttcca cgtgttcgac
      181 cagggccagt ttgccaagga ggtgctgccc aagtacttca agcacaacaa catggccagc
      241 ttcgtgcggc agctcaacat gtatggcttc cggaaagtgg tccacatcga gcagggcggc
      301 ctggtcaagc cagagagaga cgacacggag ttccagcacc catgcttcct gcgtggccag
      361 gagcagctcc ttgagaacat caagaggaaa gtgaccagtg tgtccaccct gaagagtgaa
      421 gacataaaga tccgccagga cagcgtcacc aagctgctga cggacgtgca gctgatgaag
      481 gggaagcagg agtgcatgga ctccaagctc ctggccatga agcatgagaa tgaggctctg
      541 tggcgggagg tggccagcct tcggcagaag catgcccagc aacagaaagt cgtcaacaag
      601 ctcattcagt tcctgatctc actggtgcag tcaaaccgga tcctgggggt gaagagaaag
      661 atccccctga tgctgaacga cagtggctca gcacattcca tgcccaagta tagccggcag
      721 ttctccctgg agcacgtcca cggctcgggc ccctactcgg ccccctcccc agcctacagc
      781 agctccagcc tctacgcccc tgatgctgtg gccagctctg gacccatcat ctccgacatc
      841 accgagctgg ctcctgccag ccccatggcc tcccccggcg ggagcataga cgagaggccc
      901 ctatccagca gccccctggt gcgtgtcaag gaggagcccc ccagcccgcc tcagagcccc
      961 cgggtagagg aggcgagtcc cgggcgccca tcttccgtgg acaccctctt gtccccgacc
     1021 gccctcattg actccatcct gcgggagagt gaacctgccc ccgcctccgt cacagccctc
     1081 acggacgcca ggggccacac ggacaccgag ggccggcctc cctccccccc gcccacctcc
     1141 acccctgaaa agtgcctcag cgtagcctgc ctggacaaga atgagctcag tgaccacttg
     1201 gatgctatgg actccaacct ggataacctg cagaccatgc tgagcagcca cggcttcagc
     1261 gtggacacca gtgccctgct ggacctgttc agcccctcgg tgaccgtgcc cgacatgagc
     1321 ctgcctgacc ttgacagcag cctggccagt atccaagagc tcctgtctcc ccaggagccc
     1381 cccaggcctc ccgaggcaga gaacagcagc ccggattcag ggaagcagct ggtgcactac
     1441 acagcgcagc cgctgttcct gctggacccc ggctccgtgg acaccgggag caacgacctg
     1501 ccggtgctgt ttgagctggg agagggctcc tacttctccg aaggggacgg cttcgccgag
     1561 gaccccacca tctccctgct gacaggctcg gagcctccca aagccaagga ccccactgtc
     1621 tcctacccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1681 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1741 tggctttata tatcttgtgg aaaggacgat gggtaataac tgtgagcggg gtt
//
