LOCUS       sequence-285984-        1607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3147
FEATURES             Location/Qualifiers
     source          1..1607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1439..1463)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1471..1512
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agcctggaac aaactctttt
       61 cgggtagaat ttcctgattt ttccagcacc attctacaga aactgaacca gcagcgccag
      121 caaggacaat tatgtgacgt ctccattgtt gtccaaggcc acattttccg ggcacacaaa
      181 gccgttcttg ctgccagttc accctacttt tgtgaccagg tactcctgaa aaacagcagg
      241 agaattgttt tgcctgatgt gatgaaccca agagtgtttg agaacattct cctatctagt
      301 tatacaggac gtctagtaat gcccgctcca gaaattgtta gttacttgac agcggcaagc
      361 ttcctccaga tgtggcatgt ggtagacaaa tgcactgaag ttttagaggg aaaccctaca
      421 gtcctttgtc agaagctaaa tcatggcagt gaccaccagt caccaagcag cagtagttat
      481 aatggcctgg tagagagctt tgagctgggc tctgggggtc atactgattt tcccaaagcc
      541 caagaactga gagatggtga aaatgaagag gagagcacca aagacgagct gtcatcccag
      601 ctcaccgagc acgaatacct gcccagcaac tcgtccacag agcatgaccg cctgagcacg
      661 gaaatggcaa gccaggatgg ggaggagggc gccagcgaca gcgccgagtt ccactacacc
      721 cggcccatgt acagcaagcc cagcatcatg gctcacaaac gctggatcca cgtgaagccc
      781 gagcgcttag aacaggcttg cgagggcatg gatgtgcacg cgacctacga cgagcaccag
      841 gtcacagagt ccatcaacac cgtgcagaca gagcacacgg tgcagccttc gggagtggag
      901 gaggacttcc acatcgggga gaagaaagtg gaagctgagt ttgatgaaca ggctgatgaa
      961 agcaattatg atgagcaggt ggatttctat ggctcttcca tggaagagtt ttccggagag
     1021 aggtcagatg ggaatctaat tgggcacaga caggaggctg ccctcgcagc aggttacagt
     1081 gagaatattg aaatggtaac agggattaaa gaagaagctt cccacttagg attctcagcc
     1141 accgacaagc tgtatccttg tcagtgtggg aaaagtttca ctcacaagag tcagagagat
     1201 cggcacatga gcatgcacct cggtcttcgg ccttacggct gtggggtctg cggtaagaaa
     1261 ttcaaaatga agcaccatct cgtgggccac atgaaaattc acacaggcat aaagccgtat
     1321 gagtgtaata tctgtgcaaa gaggtttatg tggagggaca gtttccaccg gcatgtgact
     1381 tcttgtacta agtcctacga agctgcaaag gctgagcaga atacaactga ggctaactac
     1441 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     1501 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1561 tatatatctt gtggaaagga cgaaacgcat accccttgtg gatttaa
//
