LOCUS       sequence-285987-        1325 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3148
FEATURES             Location/Qualifiers
     source          1..1325
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1157..1181)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1189..1230
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ccccgtgtcc tccccagcag
       61 agcaggaaca gggtgataca gctgtccact tcagagctag gagagatgga actgacttgg
      121 caggagatca tgtccatcac cgagctgcag ggtctgaatg ctccaagtga gccatcattt
      181 gagccccaag ccccagctcc ataccttgga cctccaccac ccacaactta ctgcccctgc
      241 tcaatccacc cagattctgg cttcccactt cctccaccac cttatgagct cccagcatcc
      301 acatcccatg tcccagatcc cccatactcc tatggcaaca tggccatacc agtctccaag
      361 ccactgagcc tctcaggcct gctcagtgag ccgctccaag accccttagc cctcctggac
      421 attgggctgc cagcagggcc acctaagccc caagaagacc cagaatccga ctcaggatta
      481 tccctcaact atagcgatgc tgaatctctt gagctggagg ggacagaggc tggtcggcgg
      541 cgcagcgaat atgtagagat gtacccagtg gagtacccct actcactcat gcccaactcc
      601 ttggcccact ccaactatac cttgccagct gctgagaccc ccttggcctt agagccctcc
      661 tcaggccctg tgcgggctaa gcccactgca cggggggagg cagggagtcg ggatgaacgt
      721 cgggccttgg ccatgaagat tccttttcct acggacaaga ttgtcaactt gccggtagat
      781 gactttaatg agctattggc aaggtacccg ctgacagaga gccagctagc gctagtccgg
      841 gacatccgac gacggggcaa aaacaaggtg gcagcccaga actgccgcaa gaggaagctg
      901 gaaaccattg tgcagctgga gcgggagctg gagcggctga ccaatgaacg ggagcggctt
      961 ctcagggccc gcggggaggc agaccggacc ctggaggtca tgcgccaaca gctgacagag
     1021 ctgtaccgtg acattttcca gcaccttcgg gatgaatcag gcaacagcta ctctcctgaa
     1081 gagtacgcgc tgcaacaggc tgccgatggg accatcttcc ttgtgccccg ggggaccaag
     1141 atggaggcca cagactgccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1201 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1261 atttcgattt cttggcttta tatatcttgt ggaaaggacg acatacccct gggacacatc
     1321 attga
//
