LOCUS       sequence-285993-        2186 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3150
FEATURES             Location/Qualifiers
     source          1..2186
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2018..2042)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2050..2091
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgccaggacc ccctagaagc
       61 ctagaaatgg gactgttgac atttagggat gtggccatag aattctctct ggaggagtgg
      121 caacacctgg acattgcaca gcagaattta tatagaaatg tgatgttaga gaactacaga
      181 aacctggcct tcctgggtat tgctgtctct aagccagacc tgatcacctg tctggaacaa
      241 gggaaagagc cctggaatat gaagcgacat gagatggtgg atgaaccccc aggtatgtgt
      301 cctcattttg ctcaagacct ttggccagag cagggcatgg aagattcttt tcaaaaagca
      361 atactgagaa gatatggaaa atatggacat gagaatttac agttaagaaa aggctgtaaa
      421 agtgtggatg agtataaggt gaacaaagaa ggttataatg gacttaacca gtgtttcaca
      481 actgcccaga gcaaagtatt tcaatgtgat aaatatttga aagtcttcta taaattttta
      541 aattcaaaca gacctaagat aagacatact gaaaagaaat ctttcaaatg taaaaaacgt
      601 gtcaaattat tttgcatgct ttcacataaa acccaacaca aaagcattta tcatagagag
      661 aagtcctaca aatgtaaaga atgtggaaaa acctttaatt ggtcctcaac ccttactaat
      721 cataggaaaa tttatactga agagaaacct tacaaatgtg aagaatataa caaatctcct
      781 aagcaactct caacccttac tacacatgaa ataattcatg ctggagagaa actctacaaa
      841 tgtgaagaat gtggtgaagc ttttaatcga tcctcaaatc ttactacaca taagataatt
      901 catactggag agaaacctta caagtgtgaa gaatgtggca aagcatttat ctggtcctca
      961 acccttactg agcataagaa aattcatact agaaagaaac cctacaagtg tgaagaatgt
     1021 ggcaaagcat ttatatggtc ctcaacacta actagacata agaggatgca cactggagag
     1081 aaaccctaca aatgtgaaga atgtggcaaa gcttttagcc agtcctcaac ccttactaca
     1141 cataagataa ttcatactgg agagaaacgc tacaaatgct tagaatgtgg caaagctttt
     1201 aagcaactct caactcttac tacacataaa ataattcatg ttggagagaa actctacaaa
     1261 tgtgaagaat gcggcaaagg ttttaatcga tcttcaaatc ttactacaca taagataatt
     1321 catactggag agaaacctta caagtgtgaa gaatgtggca aagcatttat ctggtcctca
     1381 acccttacta aacataagag aattcatact agagagaaac cctacaaatg tgaagaatgt
     1441 ggcaaggcat ttatatggtc ctcaacccta actagacata agaggatgca cactggagag
     1501 aaaccctaca aatgtgaaga atgtggcaaa tcttttagcc aatcctcaac ccttactaca
     1561 cataagataa ttcatactgg agagaaaccc tacaaatgtg aagaatgtgg caaagccttt
     1621 aactggtcct caactcttac taaacataag ataattcata ctgaagagaa accctacaaa
     1681 tgtgaaaaat gtggcaaagc ctttaagcag tcttcaatcc ttactaacca taagagaatt
     1741 catactggag agaaacccta taaatgtgaa gaatgtggca aatcttttaa ccggtcttca
     1801 acttttacta aacataaggt aattcatact ggagtaaaac cctacaaatg tgaagaatgt
     1861 ggcaaagcat ttttctggtc ctcaacccta actaaacata agagaattca tactggagag
     1921 caaccctaca aatgggaaaa atttggcaaa gcctttaatc ggtcctcgca cctcaccaca
     1981 gataagataa ctcattggag agaaatctta caagtattgc caactttctt gtacaaagtg
     2041 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     2101 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     2161 gatccgtgtt ggtcaagcga cgcgcc
//
