LOCUS       sequence-285999-        1715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3152
FEATURES             Location/Qualifiers
     source          1..1715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1547..1571)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1579..1620
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggggactga gaacaaggag
       61 gtgattccca aggaagaaat ttctgaagaa tctgagccac atgggtcatt attagaaaaa
      121 tttccaaaag tggtttacca aggtcatgag tttggagcag gatgtgaaga agacatgttg
      181 gagggacatt cgagagagtc catggaagag gttatagagc agatgtctcc tcaggagaga
      241 gactttccat cagggttgat gatctttaag aaatcaccct caagtgagaa agaccgggag
      301 aataatgaga gtgagagagg ctgcagtccc agcccaaatc tggttacaca tcagggagat
      361 acaacagagg gagttagtgc atttgctacc tctggccaaa acttcctaga gattttagaa
      421 tctaacaaaa cacagagaag ttctgtggga gaaaagcctc atacatgtaa agaatgtggg
      481 aaagccttta atcagaactc acatctcatc cagcatatga gagttcatag tggagaaaaa
      541 ccctttgaat gtaaagaatg tggaaagaca tttggaacta attcaagcct tcgacggcac
      601 ctgagaattc atgctggaga aaaacccttt gcttgtaatg aatgtggaaa ggccttcatt
      661 cagagttcac accttattca ccatcataga attcatactg gagagagacc ctataaatgt
      721 gaagaatgtg gtaaagcctt cagtcaaaat tcagccctta ttctacacca gagaatccat
      781 actggagaga aaccatatga atgtaatgaa tgtgggaaga cctttagggt tagttcacag
      841 cttattcagc atcagagaat tcatactgaa gaaagatacc atgaatgcaa tgagtgtggc
      901 aaagccttca agcatagctc aggccttatt agacaccaga aaattcatac tggagaaaaa
      961 ccatatctgt gtaatgaatg tgggaagggc ttcgggcaga gttctgagct tatccggcat
     1021 cagagaattc atacagggga caaaccctat gaatgtaatg aatgtgggaa aacttttggc
     1081 cagaactcag agattattag acatattaga attcatactg gtgagaagcc ctatgtatgt
     1141 aaggaatgtg ggaaggcctt cagggggaac tcagaacttc ttagacatga gagaattcac
     1201 actggagaga aaccctatga atgctttgag tgtggaaagg ctttcaggcg gacctctcac
     1261 cttattgtcc accagagaat tcatactgga gagaaacccc atcaatgtaa tgagtgtgca
     1321 agaacctttt gggataattc tgagctgctt ctccaccaga aaattcatat tggagagaaa
     1381 ccttatgaat gtagcgagtg tgagaaaaca tttagccagc attcccaact tatcatacat
     1441 cagagaattc acactggaga gaagccttat gagtgccaag aatgtcagaa gacttttagt
     1501 cggagctctc acctcctccg acatcaaagt gttcactgta tggagttgcc aactttcttg
     1561 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     1621 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1681 ggaaaggacg acgtcccaat gcgactttat aacta
//
