LOCUS       sequence-286005-        1592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3154
FEATURES             Location/Qualifiers
     source          1..1592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1424..1448)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1456..1497
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggaaaggga aggtatctgg
       61 cattctactc taggggaaac ctgggaacct aataattggt tagagggaca acaggatagt
      121 catctgagcc aagtgggagt tacccataag gaaaccttca ctgagatgag agtatgtgga
      181 ggtaatgaat ttgaaagatg ttccagtcag gattcaatcc ttgatacaca gcaaagcatt
      241 cctatggtaa aaaggcccca taactgtaat tcacatggag aagatgccac acaaaattct
      301 gagttaatta aaactcaaag aatgtttgta ggaaagaaga tctatgaatg taatcagtgc
      361 agcaaaacct tcagtcagag ctcatccctt cttaagcacc agaggattca tactggggag
      421 aaaccctata agtgtaatgt atgtgggaaa cacttcattg aacgatcctc ccttactgta
      481 catcaaagaa ttcatactgg agagaaaccc tataaatgta atgaatgtgg gaaagccttt
      541 agtcagagca tgaatcttac tgtccatcaa cgaactcaca ccggagagaa accctatcag
      601 tgtaaagagt gtggcaaagc cttccataag aattcatctc ttattcagca tgaaaggatt
      661 catactggag agaaacccta caaatgtaat gaatgtggta aagcttttac ccaaagcatg
      721 aatttgacag ttcatcagag aactcataca ggagaaaaac cctatgaatg taatgaatgt
      781 ggaaaagcct tcagtcaaag catgcatctt attgtacatc agagaagcca tactggagaa
      841 aaaccctatg agtgtagtca gtgtggaaaa gcctttagta agagctcaac tcttacccta
      901 catcagcgaa atcacactgg agaaaaacct tacaaatgta acaaatgcgg gaaatccttt
      961 agccaaagta catatcttat agaacatcag agacttcatt ctggagtaaa accttttgaa
     1021 tgtaacgagt gtggaaaagc tttcagtaag aattcatctc taactcaaca tcggagaatt
     1081 cacactggag agaaacctta tgagtgtatg gtgtgtggaa aacatttcac tggacgatca
     1141 tcccttaccg tgcatcaggt cattcacact ggagagaaac cttatgagtg caatgaatgt
     1201 ggaaaggcat tcagccagag tgcttacctt attgaacatc aaagaattca tactggtgag
     1261 aaaccctatg aatgtgatca gtgtggaaaa gccttcatta agaattcatc ccttacagtg
     1321 catcagagaa ctcatacagg agagaaaccc tatcagtgta atgaatgcgg aaaagccttc
     1381 agccggagta caaaccttac acgacatcaa agaactcata cgttgccaac tttcttgtac
     1441 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1501 taatgaacta gcccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1561 aaggacgacc aaatcacgat attttgagta ta
//
