LOCUS       sequence-286011-        1736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3156
FEATURES             Location/Qualifiers
     source          1..1736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1568..1592)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1600..1641
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acgggtttta tgatcagcaa
       61 gtccctttta tggtcccagg gaaatctcga tctgaggaat gcagagggcg gcctgtgatt
      121 gacagaaaga ggaagttttt ggacacagat ctggctcacg attctgaaga gctatttcag
      181 gatctcagtc aacttcaaga ggcttggtta gctgaagcac aagttcctga tgatgaacag
      241 tttgtcccag attttcagtc tgataacctg gtgcttcatg ccccacctcc aaccaagatc
      301 aaacgggagc tgcacagccc ctcctctgag ctgtcgtctt gtagccatga gcaggctctt
      361 ggtgctaact atggagaaaa gtgcctctac aactattgtg cctatgatag gaagcctccc
      421 tctgggttca agccattaac ccctcctaca acccccctct cacccaccca tcagaatccc
      481 ctatttcccc cacctcaggc aactctgccc acctcagggc atgcccctgc agctggccca
      541 gttcaaggtg tgggccccgc ccccgccccc cattcgcttc cagagcctgg accacagcag
      601 caaacatttg cggtcccccg accaccacat cagcccctgc agatgccaaa gatgatgcct
      661 gaaaaccagt atccatcaga acagagattt cagagacaac tgtctgaacc ctgccacccc
      721 ttccctcctc agccaggagt tcctggagat aatcgcccca gttaccatcg gcaaatgtca
      781 gaacctattg tccctgcagc tcccccgccc cctcagggat tcaaacaaga ataccatgac
      841 ccactctatg aacatggggt cccgggcatg ccagggcccc cagcacacgg gttccagtca
      901 ccaatgggaa tcaagcagga gcctcgggat tactgcgtcg attcagaagt gcctaactgc
      961 cagtcatcct acatgagagg gggttatttc tccagcagcc atgaaggttt ttcatatgaa
     1021 aaagatcccc gattatactt tgacgacact tgtgttgtgc ctgagagact ggaaggcaaa
     1081 gtcaaacagg agcctaccat gtatcgagag gggccccctt accagaggcg aggttccctt
     1141 cagctgtggc agttcctggt cacccttctt gatgacccag ccaatgccca cttcattgcc
     1201 tggacaggtc gaggcatgga gttcaagctg atagaaccgg aagaggttgc tcggcgctgg
     1261 ggcatccaga agaaccggcc agccatgaac tatgacaagc tgagccgctc tctccgctat
     1321 tactatgaaa agggcatcat gcagaaggtg gctggagagc gatacgtcta caaatttgtc
     1381 tgtgacccag atgccctctt ctccatggct ttcccggata accagcgtcc gttcctgaag
     1441 gcagagtccg agtgccacct cagcgaggag gacaccctgc cgctgaccca ctttgaagac
     1501 agccccgctt acctcctgga catggaccgc tgcagcagcc tcccctatgc cgaaggcttt
     1561 gcttactacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1621 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1681 tcttggcttt atatatcttg tggaaaggac gattcgagcc tgtggtcaga atcact
//
