LOCUS       sequence-286017-        1337 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3158
FEATURES             Location/Qualifiers
     source          1..1337
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1169..1193)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1201..1242
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgatgatgat ggcgctgagc
       61 aagaccttcg ggcagaagcc cgtgaagttc cagctggagg acgacggcga gttctacatg
      121 atcggctccg aggtgggaaa ctacctccgt atgttccgag gttctctgta caagagatac
      181 ccctcactct ggaggcgact agccactgtg gaagagagga agaaaatagt tgcatcgtca
      241 catgatcacg gatacacgac tctagccacc agtgtgaccc tgttaaaagc ctcggaagtg
      301 gaagagattc tggatggcaa cgatgagaag tacaaggctg tgtccatcag cacagagccc
      361 cccacctacc tcagggaaca gaaggccaag aggaacagcc agtgggtacc caccctgccc
      421 aacagctccc accacttaga tgccgtgcca tgctccacaa ccatcaacag gaaccgcatg
      481 ggccgagaca agaagagaac cttccccctt tgctttgatg accatgaccc agctgtgatc
      541 catgagaacg catctcagcc cgaggtgctg gtccccatcc ggctggacat ggagatcgat
      601 gggcagaagc tgcgagacgc cttcacctgg aacatgaatg agaagttgat gacgcctgag
      661 atgttttcag aaatcctctg tgacgatctg gatttgaacc cgctgacgtt tgtgccagcc
      721 atcgcctctg ccatcagaca gcagatcgag tcctacccca cggacagcat cctggaggac
      781 cagtcagacc agcgcgtcat catcaagctg aacatccatg tgggaaacat ttccctggtg
      841 gaccagtttg agtgggacat gtcagagaag gagaactcac cagagaagtt tgccctgaag
      901 ctgtgctcgg agctggggtt gggcggggag tttgtcacca ccatcgcata cagcatccgg
      961 ggacagctga gctggcatca gaagacctac gccttcagcg agaaccctct gcccacagtg
     1021 gagattgcca tccggaacac gggcgatgcg gaccagtggt gcccactgct ggagactctg
     1081 acagacgctg agatggagaa gaagatccgc gaccaggaca ggaacacgag gcggatgagg
     1141 cgtcttgcca acacggcccc ggcctggttg ccaactttct tgtacaaagt ggttgatatc
     1201 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1261 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaccattat
     1321 ggactactat atccctt
//
