LOCUS       sequence-286020-        1607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3159
FEATURES             Location/Qualifiers
     source          1..1607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1439..1463)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1471..1512
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cgtccatctt gccattcacg
       61 ccgccagttg tgaagagact gctgggatgg aagaagtcag ctggtgggtc tggaggagca
      121 ggcggaggag agcagaatgg gcaggaagaa aagtggtgtg agaaagcagt gaaaagtctg
      181 gtgaagaagc taaagaaaac aggacgatta gatgagcttg agaaagccat caccactcaa
      241 aactgtaata ctaaatgtgt taccatacca agcacttgct ctgaaatttg gggactgagt
      301 acaccaaata cgatagatca gtgggataca acaggccttt acagcttctc tgaacaaacc
      361 aggtctcttg atggtcgtct ccaggtatcc catcgaaaag gattgccaca tgttatatat
      421 tgccgattat ggcgctggcc tgatcttcac agtcatcatg aactcaaggc aattgaaaac
      481 tgcgaatatg cttttaatct taaaaaggat gaagtatgtg taaaccctta ccactatcag
      541 agagttgaga caccagtttt gcctccagta ttagtgcccc gacacaccga gatcctaaca
      601 gaacttccgc ctctggatga ctatactcac tccattccag aaaacactaa cttcccagca
      661 ggaattgagc cacagagtaa ttatattcca gaaacgccac ctcctggata tatcagtgaa
      721 gatggagaaa caagtgacca acagttgaat caaagtatgg acacaggctc tccagcagaa
      781 ctatctccta ctactctttc ccctgttaat catagcttgg atttacagcc agttacttac
      841 tcagaacctg cattttggtg ttcgatagca tattatgaat taaatcagag ggttggagaa
      901 accttccatg catcacagcc ctcactcact gtagatggct ttacagaccc atcaaattca
      961 gagaggttct gcttaggttt actctccaat gttaaccgaa atgccacggt agaaatgaca
     1021 agaaggcata taggaagagg agtgcgctta tactacatag gtggggaagt ttttgctgag
     1081 tgcctaagtg atagtgcaat ctttgtgcag agccccaatt gtaatcagag atatggctgg
     1141 caccctgcaa cagtgtgtaa aattccacca ggctgtaatc tgaagatctt caacaaccag
     1201 gaatttgctg ctcttctggc tcagtctgtt aatcagggtt ttgaagccgt ctatcagcta
     1261 actagaatgt gcaccataag aatgagtttt gtgaaagggt ggggagcaga ataccgaagg
     1321 cagacggtaa caagtactcc ttgctggatt gaacttcatc tgaatggacc tctacagtgg
     1381 ttggacaaag tattaactca gatgggatcc ccttcagtgc gttgctcaag catgtcatac
     1441 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     1501 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1561 tatatatctt gtggaaagga cgatttcggt cgaacataga cgaccgg
//
