LOCUS       sequence-286023-        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3160
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(917..941)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             949..990
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgccgcgctc cttcctggtg
       61 aaaacgcact ccagccacag ggtccccaac taccggcggc tggagacgca gagagaaatc
      121 aatggtgcct gctctgcctg tggggggctg gtggtgcccc tcctcccccg agacaaggag
      181 gccccttctg tgcccggtga ccttccccag ccctgggacc gctcctcggc cgtcgcctgc
      241 atctccctgc ccctcctgcc acggatcgag gaagctctgg gggcctctgg gctggacgcc
      301 ttggaagtca gcgaggtcga ccctcgggcc agccgggccg ccattgtacc cctcaaagac
      361 agcctgaacc acctcaacct gcccccactg ctggtgctgc ccacacggtg gtccccgacc
      421 ttgggcccag accggcacgg ggctccggaa aaactgcttg gggctgagcg gatgccccga
      481 gccccgggcg gctttgagtg cttccactgc cacaaaccct accacacgct ggccgggctg
      541 gccaggcacc ggcagctgca ctgccacctg caggtggggc gtgtcttcac ctgcaagtac
      601 tgcgacaagg agtacaccag cctgggtgcc ctcaagatgc acatccgcac tcacacgctg
      661 ccctgcacct gcaagatctg tggcaaggcc ttctccaggc cctggttact gcagggccat
      721 gtccgcaccc acacagggga gaagccctat gcctgctcgc actgcagcag ggcctttgcc
      781 gaccgctcca accttcgggc ccatctgcaa acgcactcag acgccaagaa gtaccggtgc
      841 cggcgctgca ccaagacctt ctcccgcatg tccctcctgg cgcggcatga ggagtctggc
      901 tgctgcccgg gccccttgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
      961 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1021 atttcgattt cttggcttta tatatcttgt ggaaaggacg aacttggcag ctgttagttg
     1081 ccttt
//
