LOCUS       sequence-286026-        2120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3161
FEATURES             Location/Qualifiers
     source          1..2120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1952..1976)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1984..2025
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc aagaaacctt caggaacctg
       61 gcctctatag ggaaaaaatg gaaaccccag aacatatatg tagagtacga aaatctaagg
      121 agaaacctaa gaattgtggg agagagactc tttgaaagta aagaaggtca tcagcatgga
      181 gaaattttga cccaggttcc agatgacatg ctgaagaaaa caactactgg agtaaaatca
      241 tgcgaaagca gtgtgtatgg agaagtaggc agtgctcatt catctcttaa taggcacatc
      301 agagatgaca ctggacacaa ggcatatgag tatcaagaat atggacagaa accatataaa
      361 tgtaaatact gtaaaaaacc tttcaactgt ctctcctctg ttcagacaca tgaaagggct
      421 catagtggaa ggaaactcta tgtttgtgag gaatgcggaa aaacatttat ttcccattca
      481 aaccttcaaa gacacaggat aatgcaccgt ggagatggac cttataagtg taaattttgt
      541 gggaaagcct tgatgtttct cagtttgtat cttatccaca aacgaactca cactggagag
      601 aaaccatatc aatgtaaaca gtgtggtaaa gcctttagtc attctagtag ccttcgaata
      661 catgaaagaa ctcacactgg ggagaagcct tataaatgta atgaatgtgg gaaagcattc
      721 catagttcca catgccttca tgctcataaa agaactcaca ctggggagaa gccatatgaa
      781 tgtaaacagt gtgggaaagc cttcagctct tcccattcct ttcaaataca tgaaagaact
      841 cacacggggg agaagccata tgaatgtaag gaatgtggaa aagcattcaa gtgtcccagt
      901 tctgttcgca gacatgaaag aacccactct aggaaaaaac cctatgaatg taaacattgt
      961 gggaaagtat tatcttatct taccagcttt caaaaccact tgggaatgca cactggagag
     1021 atatctcata aatgtaagat atgtgggaaa gccttttatt ctcccagttc acttcaaaca
     1081 catgaaaaaa ctcacactgg agagaaaccc tataaatgca accaatgtgg taaagccttt
     1141 aattcttcca gttccttccg atatcatgaa agaactcaca ctggagagaa accttacgag
     1201 tgtaagcaat gtgggaaagc cttcagatct gcctcactcc ttcaaacaca tggtaggact
     1261 cacacgggag agaaacccta tgcatgtaag gaatgtggaa aaccatttag taatttctct
     1321 ttctttcaaa tacatgaaag gatgcacaga gaagagaagc cgtatgaatg taagggttat
     1381 gggaaaacat tcagtttgcc cagtttattt catagacatg aaaggactca cactggagga
     1441 aaaacctatg aatgcaagca gtgtggcaga tccttcaact gttcgagctc ctttcgatat
     1501 catggaagga ctcacactgg agagaaaccc tatgaatgca agcaatgtgg aaaagccttc
     1561 agatctgcct cacagcttca aattcatgga aggactcaca ctggagagaa accttatgaa
     1621 tgtaagcagt gtgggaaagc ctttggatct gcctcacacc ttcaaatgca tggaaggact
     1681 cacactggag agaaacccta tgaatgtaag cagtgtggga agtcttttgg atgtgcctcg
     1741 cgacttcaaa tgcatggaag gactcacact ggagagaaac cgtataaatg taagcaatgt
     1801 gggaaagctt ttggatgtcc ctcaaacctt cgaaggcatg gaaggactca cactggagag
     1861 aaaccctata aatgtaacca atgtggtaaa gtctttagat gttcttcaca acttcaagtg
     1921 catggaaggg ctcactgcat agacacccca tacccaactt tcttgtacaa agtggttgat
     1981 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     2041 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaagat
     2101 gagacaggcc ttgccatgga
//
