LOCUS       sequence-286029-        1475 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3162
FEATURES             Location/Qualifiers
     source          1..1475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1307..1331)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1339..1380
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gttctcattt tgcccaagac
       61 ctttggccag agaacataca aaattctttc caaataggga tgctgagaag atatgaagaa
      121 tgcagacatg acaatttaca gttaaaaaaa ggctgtaaaa gcgtgggtga gcataaggtg
      181 cacaaaggag gttataatgg acttaaccaa tgtttgacaa ctacccagaa agaaatattt
      241 caatgtgata aatatggaaa agtctttcat aagttttcaa attcaaacac atataagaca
      301 agacatactg gaataaatct tttcaaatgt ataatatgtg gcaaagcttt taaacggtcc
      361 tcaaccctta ctacacataa gaaaattcat actggagaga aaccttacag atgtgaagaa
      421 tgtggcaaag cttttaacca atctgcaaac cttactacac ataagagaat tcataccgga
      481 gagaaaccct acagatgtga agaatgtggc aaagccttta agcagtcctc aaaccttact
      541 acacataaga aaattcatac tggagagaaa ccctacaaat gtgaagaatg tggcaaagcc
      601 ttcaaccgat ccacagacct tactacacat aagatagttc atactggaga gaaaccctac
      661 aaatgtgaag aatgtggcaa agcctttaag cacccctcac acgttaccac acataagaaa
      721 attcatacta gagggaaacc ctacaactgt gaagaatgtg gcaaatcctt taagcactgc
      781 tctaacctta ctatacataa gagaattcat acaggagaga aaccctacaa atgtgaagaa
      841 tgtggcaaag cctttcacct atcctcacac cttactacac ataagatact tcatactgga
      901 gagaaaccct acagatgtag agaatgtggc aaagctttta accattccac aacccttttt
      961 tcacatgaga aaattcatac tggagagaaa ccctacaaat gtgatgaatg tggcaaaacc
     1021 tttacctggc cctcaatcct ctccaaacat aaaagaactc atactggaga gaaaccctac
     1081 aaatgtgaag aatgtggcaa atcctttact gcatcctcaa ctctaactac acataagaga
     1141 attcatactg gagagaaacc ttacaaatgt gaagaatgtg gcaaagcttt taactggtcc
     1201 tcagacctta ataaacataa gaaaattcat attgaacgaa aaccctacat agtgaagaat
     1261 gtgacagatc ttttaaatgt tcctccactt ttaattagca taagataccc aactttcttg
     1321 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     1381 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1441 ggaaaggacg acaaatctct gttgaccaga cggtg
//
