LOCUS       sequence-286032-        3479 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3163
FEATURES             Location/Qualifiers
     source          1..3479
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3311..3335)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3343..3384
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgatgccgat gatattaact
       61 gttttcttga gcaacaatga acagatttta acagaagttc ctataacacc ggaaacaacc
      121 tgtcgagatg ttgtagaatt ttgcaaggaa cctggagaag gcagctgcca tttagctgaa
      181 gtgtggaggg gaaatgaacg tcccataccc tttgatcata tgatgtacga acatcttcag
      241 aaatggggtc cacggaggga agaagtgaaa tttttccttc gacacgagga ctccccaact
      301 gagaacagtg aacaaggtgg ccgtcagacc caagagcaac gaactcagag aaatgtaata
      361 aatgtacctg gagaaaaacg tactgaaaat ggggttggga atccacgtgt tgaacttacc
      421 ctctcagagc tccaagatat ggcagctagg caacagcagc agattgaaaa tcagcagcag
      481 atgttggttg ccaaggaaca gcgtttacat tttctaaagc aacaggagcg ccgtcagcag
      541 cagtctattt ctgaaaatga aaagcttcag aaattgaaag aacgagttga agcccaggag
      601 aacaagctga agaaaattcg tgcaatgaga ggacaagtcg actacagcaa aatcatgaac
      661 ggcaatctgt ctgctgaaat agaaaggttc agtgccatgt tccaggaaaa gaagcaggaa
      721 gtacagactg caattttaag ggttgatcag cttagtcagc aattggaaga tttaaagaaa
      781 ggaaaactga atgggttcca gtcttacaat ggcaaattga cgggaccagc ggcggtggag
      841 ttaaaaagac tgtaccaaga actacagatt cgtaaccaac ttaaccagga acaaaattca
      901 aaacttcagc agcagaagga actcttaaat aagcgcaaca tggaggtggc catgatggac
      961 aagcgaatca gtgaactgcg tgaacgtctc tatgggaaaa aaattcagct gaaccgtgtg
     1021 aatggcacgt catcaccaca gtcccctctg agcacatcgg gcagggtcgc tgctgtgggg
     1081 ccttatatcc aggttcccag tgccggaagc tttcctgtgc tgggggaccc tataaagccc
     1141 cagtctctca gtattgcctc aaatgctgct catggaagat ccaaatccgc taatgatgga
     1201 aactggccaa cattaaaaca gaattctagc tcttccgtga aaccagtgca ggtggccggt
     1261 gcagactgga aggatccgag cgtggagggg tctgtcaagc agggcactgt ctccagccag
     1321 cctgtgccct tctcagcact gggacccacg gagaagccgg gcatcgagat tggtaaagtg
     1381 ccacctccca tcccgggtgt aggcaagcag ctgcctccaa gctatgggac atacccaagt
     1441 cctacacctc tgggtcctgg gtcgacaagc tccctggaaa ggaggaagga aggcagcttg
     1501 cccaggccca gtgcaggcct gccaagtcga cagaggccca ccctgctgcc cgccacaggc
     1561 agcacccccc agccaggctc ctcacaacag attcagcaga ggatttccgt accgccaagt
     1621 cccacgtacc cgccagcggg accacctgca tttccagctg gggacagcaa gcctgaactc
     1681 ccactgacag tggccattag gcctttcctg gctgataaag ggtcaaggcc acagtctccc
     1741 aggaaaggac cccagacagt gaattcaagt tccatatact ccatgtacct ccagcaagcc
     1801 acaccaccta agaattacca gccggcagca cacagcgcct taaataagtc agttaaagca
     1861 gtgtatggta agcccgtttt accttcgggt tcaacctctc catcgccgct gccgtttctt
     1921 cacgggtcac tgtccacggg cacaccacag cctcagccac cttcagaaag tactgagaaa
     1981 gagcctgagc aggatggccc cgccgccccc gcagatggca gcaccgtgga gagcctgcca
     2041 cggccactca gccccaccaa gctcacgccc atcgtgcatt cgccactgcg ctaccagagt
     2101 gatgcagacc tggaggccct ccgcaggaag ctggccaacg cgccccggcc cctgaaaaag
     2161 cgcagctcca tcacagagcc cgagggcccc ggcgggccca acatccagaa gctgctgtac
     2221 cagcgcttca acaccctggc cggtggcatg gagggcaccc ctttctacca gcccagcccc
     2281 tcccaggact tcatgggcac cttggccgat gtggacaatg gaaacaccaa tgccaatgga
     2341 aacctggaag agctcccccc tgcccagccc acagccccac tccccgctga gcctgccccg
     2401 tcatcagatg ccaatgataa tgagttacct tcccccgaac cagaggagct catctgtccc
     2461 caaaccaccc accaaactgc cgagccggca gaggacaata acaacaacgt ggccacggtc
     2521 cccaccacgg agcagatccc gagtcctgtg gctgaggccc catctccagg ggaagagcag
     2581 gtccctccag cacctcttcc ccctgccagc caccctcctg ccacctccac gaacaagcgg
     2641 accaacttga agaagcccaa ctcggagcgg acggggcacg ggctgagagt ccggtttaac
     2701 cccctggcac tgctcctaga cgcgtctctg gaaggagagt tcgatctggt gcagaggatc
     2761 atctatgagg tggaagatcc cagcaagccc aacgacgaag ggatcacccc actgcacaac
     2821 gccgtctgcg ccggccacca tcacatcgtg aagttcctgc tggattttgg tgtcaacgtg
     2881 aatgctgctg atagtgatgg atggacgccg ctgcactgcg ctgcctcttg taacagcgtt
     2941 cacctctgca aacagctggt ggagagtggt gccgccattt ttgcctcaac cataagcgac
     3001 attgaaactg ctgcagacaa gtgtgaggag atggaggaag gctacatcca gtgctcccag
     3061 tttctatatg gggtgcagga aaagctgggt gtgatgaaca aaggtgtggc gtatgctctg
     3121 tgggactacg aggcccagaa cagtgacgag ctgtccttcc acgaagggga cgccctcacc
     3181 atcctgaggc gcaaggacga aagcgagact gagtggtggt gggctcgcct tggagaccgg
     3241 gagggctatg tgcccaaaaa cctgctgggg ctgtatccac ggatcaaacc ccgacagcga
     3301 acactcgcct tgccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     3361 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     3421 atttcttggc tttatatatc ttgtggaaag gacgaactgc cactgataaa ctaccgcgt
//
