LOCUS       sequence-286035-        1573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3164
FEATURES             Location/Qualifiers
     source          1..1573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1406..1430)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1438..1479
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga agattgagga ggtgaagagc
       61 actacgaaga cgcagcgcat cgcctcccac agccacgtga aagggctggg gctggacgag
      121 agcggcttgg ccaagcaggc ggcctcaggg cttgtgggcc aggagaacgc gcgagaggca
      181 tgtggcgtca tagtagaatt aatcaaaagc aagaaaatgg ctggaagagc tgtcttgttg
      241 gcaggacctc ctggaactgg caagacagct ctggctctgg ctattgctca ggagctgggt
      301 agtaaggtcc ccttctgccc aatggtgggg agtgaagttt actcaactga gatcaagaag
      361 acagaggtgc tgatggagaa cttccgcagg gccattgggc tgcgaataaa ggagaccaag
      421 gaagtttatg aaggtgaagt cacagagcta actccgtgtg agacagagaa tcccatggga
      481 ggatatggca aaaccattag ccatgtgatc ataggactca aaacagccaa aggaaccaaa
      541 cagttgaaac tggaccccag catttttgaa agtttgcaga aagagcgagt agaagctgga
      601 gatgtgattt acattgaagc caacagtggg gccgtgaaga ggcagggcag gtgtgatacc
      661 tatgccacag aattcgacct tgaagctgaa gagtatgtcc ccttgccaaa aggggatgtg
      721 cacaaaaaga aagaaatcat ccaagatgtg accttgcatg acttggatgt ggctaatgcg
      781 cggccccagg ggggacaaga tatcctgtcc atgatgggcc agctaatgaa gccaaagaag
      841 acagaaatca cagacaaact tcgaggggag attaataagg tggtgaacaa gtacatcgac
      901 cagggcattg ctgagctggt cccgggtgtg ctgtttgttg atgaggtcca catgctggac
      961 attgagtgct tcacctacct gcaccgcgcc ctggagtctt ctatcgctcc catcgtcatc
     1021 tttgcatcca accgaggcaa ctgtgtcatc agaggcactg aggacatcac atcccctcac
     1081 ggcatccctc ttgaccttct ggaccgagtg atgataatcc ggaccatgct gtatactcca
     1141 caggaaatga aacagatcat taaaatccgt gcccagacgg aaggaatcaa catcagtgag
     1201 gaggcactga accacctggg ggagattggc accaagacca cactgaggta ctcagtgcag
     1261 ctgctgaccc cggccaactt gcttgctaaa atcaacggga aggacagcat tgagaaagag
     1321 catgtcgaag agatcagtga acttttctat gatgccaagt cctccgccaa aatcctggct
     1381 gaccagcagg ataagtacat gaagtgccca actttcttgt acaaagtggt tgatatcggt
     1441 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1501 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat cagagcaata
     1561 tcccataatc agt
//
