LOCUS       sequence-286038-        2711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3165
FEATURES             Location/Qualifiers
     source          1..2711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2543..2567)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2575..2616
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggcctccgc ggcggcagcc
       61 tcggcagcag cggcctcggc cgcctctggc agcccgggcc cgggcgaggg ctccgctggc
      121 ggcgaaaagc gctccaccgc cccttcggcc gcagcctcgg cctctgcctc agccgcggcg
      181 tcgtcgcccg cggggggcgg cgccgaggcg ctggagctgc tggagcactg cggcgtgtgc
      241 agagagcgcc tgcgacccga gagggagccc cgcctgctgc cctgtttgca ctcggcctgt
      301 agtgcctgct tagggcccgc ggcccccgcc gccgccaaca gctcggggga cggcggggcg
      361 gcgggcgacg gcaccgtggt ggactgtccc gtgtgcaagc aacagtgctt ctccaaagac
      421 atcgtggaga attatttcat gcgtgatagt ggcagcaagg ctgccaccga cgcccaggat
      481 gcgaaccagt gctgcactag ctgtgaggat aatgccccag ccaccagcta ctgtgtggag
      541 tgctcggagc ctctgtgtga gacctgtgta gaggcgcacc agcgggtgaa gtacaccaag
      601 gaccatactg tgcgctctac tgggccagcc aagtctcggg atggtgaacg tactgtctat
      661 tgcaacgtac acaagcatga accccttgtg ctgttttgtg agagctgtga tactctcacc
      721 tgccgagact gccagctcaa tgcccacaag gaccaccagt accagttctt agaggatgca
      781 gtgaggaacc agcgcaagct cctggcctca ctggtgaagc gccttgggga caaacatgca
      841 acattgcaga agagcaccaa ggaggttcgc agctcaatcc gccaggtgtc tgacgtacag
      901 aagcgtgtgc aagtggatgt caagatggcc atcctgcaga tcatgaagga gctgaataag
      961 cggggccgtg tgctggtcaa tgatgcccag aaggtgactg aggggcagca ggagcgcctg
     1021 gagcggcagc actggaccat gaccaagatc cagaagcacc aggagcacat tctgcgcttt
     1081 gcctcttggg ctctggagag tgacaacaac acagcccttt tgctttctaa gaagttgatc
     1141 tacttccagc tgcaccgggc cctcaagatg attgtggatc ccgtggagcc acatggcgag
     1201 atgaagtttc agtgggacct caatgcctgg accaagagtg ccgaggcctt tggcaagatt
     1261 gtggcagagc gtcctggcac taactcaaca ggccctgcac ccatggcccc tccaagagcc
     1321 ccagggcccc tgagcaagca gggctctggc agcagccagc ccatggaggt gcaggaaggc
     1381 tatggctttg ggtcaggaga tgatccctac tcaagtgcag agccccatgt gtcaggtgtg
     1441 aaacggtccc gctcaggtga gggcgaggtg agcggcctta tgcgcaaggt gccacgagtg
     1501 agccttgaac gcctggacct ggacctcaca gctgacagcc agccacccgt cttcaaggtc
     1561 ttcccaggca gtaccactga ggactacaac cttattgtta ttgaacgtgg cgctgccgct
     1621 gcagctaccg gccagccagg gactgcgcct gcaggaaccc ctggtgcccc acccctggct
     1681 ggcatggcca ttgtcaagga ggaggagacg gaggctgcca ttggagcccc tcctactgcc
     1741 actgagggcc ctgagaccaa acctgtgctt atggctcttg cggagggtcc tggtgctgag
     1801 ggtccccgcc tggcctcacc tagtggcagc accagctcag ggctggaggt ggtggctcct
     1861 gagggtacct cagccccagg tggtggcccg ggaaccctgg atgacagtgc caccatttgc
     1921 cgtgtctgcc agaagccagg cgatctggtt atgtgcaacc agtgtgagtt ttgtttccac
     1981 ctggactgtc acctgccggc cctgcaggat gtaccagggg aggagtggag ctgctcactc
     2041 tgccatgtgc tccctgacct gaaggaggag gatggcagcc tcagcctgga tggtgcagac
     2101 agcactggcg tggtggccaa gctctcacca gccaaccagc ggaaatgtga gcgtgtactg
     2161 ctggccctat tctgtcacga accctgccgc cccctgcatc agctggctac cgactccacc
     2221 ttctccctgg accagcccgg tggcaccctg gatctgaccc tgatccgtgc ccgcctccag
     2281 gagaagttgt cacctcccta cagctcccca caggagtttg cccaggatgt gggccgcatg
     2341 ttcaagcaat tcaacaagtt aactgaggac aaggcagacg tgcagtccat catcggcctg
     2401 cagcgcttct tcgagacgcg catgaacgag gccttcggtg acaccaagtt ctctgctgtg
     2461 ctggtggagc ccccgccgat gagcctgcct ggtgctggcc tgagttccca ggagctgtct
     2521 ggtggccctg gtgatggccc ctgcccaact ttcttgtaca aagtggttga tatcggtaag
     2581 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     2641 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacga aatgcccata
     2701 cagctccccc g
//
