LOCUS       sequence-286041-        2885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3166
FEATURES             Location/Qualifiers
     source          1..2885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2717..2741)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2749..2790
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgacggatcc tttctgcgtt
       61 ggaggccgtc ggctcccggg gtccagcaag agtggtcccg ggaaggatgg cagccgaaag
      121 gaggtccgac ttccaatgct gcatgaccca ccgaagatgg ggatgccggt ggtccgtggt
      181 ggacagacag tgcccggcca ggcccctctc tgctttgacc cgggaagtcc agccagtgac
      241 aagacagaag ggaagaaaaa ggggcggcca aaagccgaga accaggccct ccgagacatt
      301 cctctctccc tgatgaacga ctggaaggat gagttcaagg cacactcgag ggtgaagtgt
      361 ccaaactcag ggtgctggct ggagttcccc agcatctacg ggctcaagta ccattaccag
      421 cggtgccaag ggggtgccat ctcagatcgc ctggccttcc cctgcccctt ctgcgaggcc
      481 gcattcacct ctaagaccca gctggagaaa caccggatct ggaaccacat ggaccgaccc
      541 ctgcctgcct ccaagcctgg gcccatcagc aggccggtca ccatcagccg gcctgttggg
      601 gtcagcaagc ccatcggagt gagcaaacct gtcactattg gcaaacctgt gggtgtcagc
      661 aaacccattg gcatcagcaa gccagtctcg gtcggcagac ccatgccagt caccaaggcc
      721 atcccggtca ctaggcccgt gccagtcacc aaacctgtca cagtcagcag gcccatgccc
      781 gtcaccaagg ccatgccggt caccaaaccc atcacagtca ccaagtctgt gccggtcacc
      841 aaacccgtac ctgtcaccaa acccattacg gtaacaaagc ttgtgacagt tacgaaaccc
      901 gtgccggtca ccaagccagt gacagtcagc aggcccattg tggtcagcaa gccggtgaca
      961 gtcagcaggc ccattgctat cagcagacac acaccgccct gcaaaatggt gctgctgacc
     1021 aggtcggaga acaaagcacc tcgtgccaca gggaggaaca gtggtaagaa aagggctgcg
     1081 gacagcctgg acacctgccc aattccaccc aagcaggcca ggccagagaa tggggagtac
     1141 ggcccctcct ccatgggcca gagctcggcc ttccagctga gtgcagacac cagcagtggc
     1201 tccttgtcgc caggcagcag gccgtcaggg ggcatggagg cactgaaggc tgcaggccct
     1261 gcgtccccgc ctgaggagga cccggagcgc acaaagcaca gaaggaaaca gaaaacaccc
     1321 aaaaagttta caggggagca gccatccatc tccgggacct ttgggctcaa aggcctggtc
     1381 aaagctgagg acaaggcccg agttcaccgc tccaagaagc aggaggggcc aggccctgag
     1441 gacgcccgga agaaggtgcc agctgccccc atcactgtca gcaaggaggc accggcccct
     1501 gtggcccacc cagctccagg tggccctgaa gagcagtggc agagggccat ccatgagcgc
     1561 ggggaagccg tctgccccac ctgcaacgtg gtcacccgga agactctcgt ggggcttaag
     1621 aagcacatgg aggtgtgtca gaagcttcag gatgcactca agtgccagca ctgccggaag
     1681 cagttcaagt ccaaagccgg cctcaactac cacactatgg ccgagcacag tgccaagccc
     1741 tctgacgccg aggcctccga agggggcgag caggaggagc gcgagaggct gcgcaaggtg
     1801 ctgaagcaga tgggacggct gcgctgcccc caggagggtt gcggggctgc cttctccagc
     1861 ctcatgggct accagtacca ccagcggcgc tgcgggaagc cgccctgcga ggtggacagc
     1921 ccctccttcc cctgcaccca ctgtggcaag acgtaccgat ccaaggctgg ccacgactac
     1981 cacgtgcgct cggagcacac ggcccccccc cctgaggagc ccacagacaa gtcccctgag
     2041 gctgaggacc cgctgggtgt ggagcggacc ccaagcgggc gtgtccgccg cacgtcggcc
     2101 caggtggcgg tgttccacct gcaggagata gcggaggacg agctggcccg cgactggacc
     2161 aagcggcgca tgaaggatga ccttgtgccc gagaccgcac ggctcaacta cactcgacca
     2221 gggctcccca cgctgaaccc ccagctgcta gaggcatgga agaatgaagt gaaggagaaa
     2281 ggccacgtca actgtcccaa cgactgctgt gaagccatct actccagcgt gtccggactc
     2341 aaggctcatc tcgccagctg cagtaagggg gcccacctgg cagggaagta ccgctgtctg
     2401 ctgtgtccga aggagttcag ttctgagagt ggcgtcaaat accacatcct gaagacccac
     2461 gcagagaact ggttccgaac atcagcagac ccacctccca aacacaggag ccaggactca
     2521 ttggtgccca agaaggaaaa gaagaaaaat ctggcaggtg gaaagaagcg gggccgaaag
     2581 cccaaggagc ggaccccaga ggagcctgtg gccaagctgc ccccgcgccg ggacgactgg
     2641 cctccaggat gcagagacaa gggggcccgg ggctccaccg gccggaaggt gggagtcagc
     2701 aaggcgcctg aaaagttgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     2761 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     2821 atttcgattt cttggcttta tatatcttgt ggaaaggacg agctggtgtt tccaaggcat
     2881 acgtt
//
