LOCUS       sequence-286047-        2495 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3168
FEATURES             Location/Qualifiers
     source          1..2495
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             1354..1365
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(2327..2351)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2359..2400
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga aaactagccc ccgtcggcca
       61 ctgattctca aaagacggag gctgcccctt cctgttcaaa atgccccaag tgaaacatca
      121 gaggaggaac ctaagagatc ccctgcccaa caggagtcta atcaagcaga ggcctccaag
      181 gaagtggcag agtccaactc ttgcaagttt ccagctggga tcaagattat taaccacccc
      241 accatgccca acacgcaagt agtggccatc cccaacaatg ctaatattca cagcatcatc
      301 acagcactga ctgccaaggg aaaagagagt ggcagtagtg ggcccaacaa attcatcctc
      361 atcagctgtg ggggagcccc aactcagcct ccaggactcc ggcctcaaac ccaaaccagc
      421 tatgatgcca aaaggacaga agtgaccctg gagaccttgg gaccaaaacc tgcagctagg
      481 gatgtgaatc ttcctagacc acctggagcc ctttgcgagc agaaacggga gacctgtgca
      541 gatggtgagg cagcaggctg cactatcaac aatagcctat ccaacatcca gtggcttcga
      601 aagatgagtt ctgatggact gggctcccgc agcatcaagc aagagatgga ggaaaaggag
      661 aattgtcacc tggagcagcg acaggttaag gttgaggagc cttcgagacc atcagcgtcc
      721 tggcagaact ctgtgtctga gcggccaccc tactcttaca tggccatgat acaattcgcc
      781 atcaacagca ctgagaggaa gcgcatgact ttgaaagaca tctatacgtg gattgaggac
      841 cactttccct actttaagca cattgccaag ccaggctgga agaactccat ccgccacaac
      901 ctttccctgc acgacatgtt tgtccgggag acgtctgcca atggcaaggt ctccttctgg
      961 accattcacc ccagtgccaa ccgctacttg acattggacc aggtgtttaa gccactggac
     1021 ccagggtctc cacaattgcc cgagcacttg gaatcacagc agaaacgacc gaatccagag
     1081 ctccgccgga acatgaccat caaaaccgaa ctccccctgg gcgcacggcg gaagatgaag
     1141 ccactgctac cacgggtcag ctcatacctg gtacctatcc agttcccggt gaaccagtca
     1201 ctggtgttgc agccctcggt gaaggtgcca ttgcccctgg cggcttccct catgagctca
     1261 gagcttgccc gccatagcaa gcgagtccgc attgccccca aggtgctgct agctgaggag
     1321 gggatagctc ctctttcttc tgcaggacca gggaaagagg agaaactcct gtttggagaa
     1381 gggttttctc ctttgcttcc agttcagact atcaaggagg aagaaatcca gcctggggag
     1441 gaaatgccac acttagcgag acccatcaaa gtggagagcc ctcccttgga agagtggccc
     1501 tccccggccc catctttcaa agaggaatca tctcactcct gggaggattc gtcccaatct
     1561 cccaccccaa gacccaagaa gtcctacagt gggcttaggt ccccaacccg gtgtgtctcg
     1621 gaaatgcttg tgattcaaca cagggagagg agggagagga gccggtctcg gaggaaacag
     1681 catctactgc ctccctgtgt ggatgagccg gagctgctct tctcagaggg gcccagtact
     1741 tcccgctggg ccgcagagct cccgttccca gcagactcct ctgaccctgc ctcccagctc
     1801 agctactccc aggaagtggg aggacctttt aagacaccca ttaaggaaac gctgcccatc
     1861 tcctccaccc cgagcaaatc tgtcctcccc agaacccctg aatcctggag gctcacgccc
     1921 ccagccaaag tagggggact ggatttcagc ccagtacaaa cctcccaggg tgcctctgac
     1981 cccttgcctg accccctggg gctgatggat ctcagcacca ctcccttgca aagtgctccc
     2041 ccccttgaat caccgcaaag gctcctcagt tcagaaccct tagacctcat ctccgtcccc
     2101 tttggcaact cttctccctc agatatagac gtccccaagc caggctcccc ggagccacag
     2161 gtttctggcc ttgcagccaa tcgttctctg acagaaggcc tggtcctgga cacaatgaat
     2221 gacagcctca gcaagatcct gctggacatc agctttcctg gcctggacga ggacccactg
     2281 ggccctgaca acatcaactg gtcccagttt attcctgagc tacagtaccc aactttcttg
     2341 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     2401 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2461 ggaaaggacg acacggcgcg taggactcgt ggcaa
//
