LOCUS       sequence-286053-        2465 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3170
FEATURES             Location/Qualifiers
     source          1..2465
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2297..2321)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2329..2370
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggacaagcg caaactccaa
       61 ggtgagattg atcgctgcct caagaaggtg tccgagggcg tggagcagtt tgaagatatt
      121 tggcagaagc tccacaatgc agccaacgcg aaccagaaag aaaagtatga ggctgaccta
      181 aagaaggaga ttaagaagct acaacggctg agggaccaaa tcaagacatg ggtagcgtcc
      241 aacgagatca aggacaagag gcagcttata gacaaccgca agctcattga gacgcaaatg
      301 gaacggttca aagttgtgga acgagagacc aaaaccaaag cttacagcaa agagggcctg
      361 ggcctggccc agaaggtaga tcctgcccag aaggagaagg aagaggttgg ccagtggctc
      421 acgaatacca tcgacacgct caacatgcag gtggaccagt ttgagagtga agtggagtca
      481 ctgtcagtgc agacacgcaa gaagaagggc gacaaggata agcaggaccg gattgagggc
      541 ttgaagcggc acatcgagaa gcaccgctac cacgtgcgca tgctagagac catcctgcgc
      601 atgctggaca atgactccat cctcgttgac gccatccgca agatcaagga cgacgttgag
      661 tactatgttg actcatccca ggaccccgac ttcgaggaga acgagtttct ctacgatgac
      721 ctggacctcg aggacattcc acaggcgctg gtcgccacct ccccccccag ccacagccac
      781 atggaggatg agatcttcaa ccagtccagc agcacgccca cctcaaccac ctccagctct
      841 cccatcccgc ccagcccagc caactgtacc acggaaaact ctgaagatga taagaagagg
      901 ggacgttcca cagacagtga agtcagccag tctccagcca aaaacggctc caagcctgtc
      961 cacagcaacc agcaccctca gtccccagct gtgccgccca cctacccctc cggccccccg
     1021 cctgctgcct ctgccttgag caccactcct ggcaacaatg gggtccccgc ccccgcagca
     1081 cccccaagtg ccctgggccc caaggccagt ccagctccca gccacaactc gggcacccct
     1141 gctccctatg cccaggcggt ggccccacca gctcccagtg ggcccagcac gacccagccc
     1201 cggcccccca gcgtccagcc tagcggaggc ggaggcggcg gcagcggagg cggagggagc
     1261 agcagcagta gtaacagcag tgccggtgga ggggctggca agcagaatgg cgccaccagt
     1321 tacagctcag ttgtggcaga cagcccggca gaggtggctt tgagcagcag tgggggcaac
     1381 aatgccagca gccaggcctt gggcccccct tccggccccc acaacccacc tcccagcacc
     1441 tcgaaggaac ccagtgcggc agccccaacg ggggctgggg gcgtggcccc aggctcaggg
     1501 aacaactcag ggggacccag cctcctggtg ccactgcctg tgaatcctcc cagctcccca
     1561 acgcccagct tcagtgatgc caaggcagcc ggtgccctgc tcaatgggcc tccacagttc
     1621 agcaccgccc cagaaatcaa ggcccctgag cctctgagct ccttgaagtc catggcggaa
     1681 cgggcagcca tcagctctgg cattgaggac cctgtgccaa cgctgcacct gaccgagcga
     1741 gacatcatcc tgagcagtac atcagcacct ccggcctcag cccagccgcc cctgcagctg
     1801 tcagaggtga acataccgct gtcgctgggt gtctgtccac tgggccctgt gcccctcacc
     1861 aaggagcagc tctatcagca ggccatggaa gaggccgcct ggcaccacat gcctcacccc
     1921 tctgactctg agcgtattcg gcagtacctc ccccggaacc cctgtccgac gcccccctac
     1981 caccaccaga tgccaccccc acactcggac actgtggaat tctaccagcg cctgtcgacc
     2041 gagacactct tcttcatctt ctactatctg gagggcacta aggcacagta tctggcagcc
     2101 aaggccctaa agaagcagtc atggcgattc cacaccaagt acatgatgtg gttccagagg
     2161 cacgaggagc ccaagaccat cactgacgag tttgagcagg gcacctacat ctactttgac
     2221 tacgagaagt ggggccagcg gaagaaggaa ggcttcacct ttgagtaccg ctacctggag
     2281 gaccgggacc tccagtgccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     2341 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     2401 atttcgattt cttggcttta tatatcttgt ggaaaggacg attcgttgta ctggatttcg
     2461 gactc
//
