LOCUS       sequence-286062-        2753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3173
FEATURES             Location/Qualifiers
     source          1..2753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2585..2609)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2617..2658
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tgtctctgtg gggtctggtc
       61 tccaagatgc ccccagaaaa agtgcagcgg ctctatgtcg actttcccca acacctgcgg
      121 catcttctgg gtgactggct ggagagccag ccctgggagt tcctggtcgg ctccgacgcc
      181 ttctgctgca acttggctag tgccctactt tcagacactg tccagcacct tcaggcctcg
      241 gtgggagagc agggggaggg gagcaccatc ttgcaacaca tcagcaccct tgagagcata
      301 tatcagaggg accccctgaa gctggtggcc actttcagac aaatacttca aggagagaaa
      361 aaagctgtta tggaacagtt ccgccacttg ccaatgcctt tccactggaa gcaggaagaa
      421 ctcaagttta agacaggctt gcggaggctg cagcaccgag taggggagat ccaccttctc
      481 cgagaagccc tgcagaaggg ggctgaggct ggccaagtgt ctctgcacag cttgatagaa
      541 actcctgcta atgggactgg gccaagtgag gccctggcca tgctactgca ggagaccact
      601 ggagagctag aggcagccaa agccctagtg ctgaagagga tccagatttg gaaacggcag
      661 cagcagctgg cagggaatgg cgcaccgttt gaggagagcc tggccccact ccaggagagg
      721 tgtgaaagcc tggtggacat ttattcccag ctacagcagg aggtaggggc ggctggtggg
      781 gagcttgagc ccaagacccg ggcatcgctg actggccggc tggatgaagt cctgagaacc
      841 ctcgtcacca gttgcttcct ggtggagaag cagccccccc aggtactgaa gactcagacc
      901 aagttccagg ctggagttcg attcctgttg ggcttgaggt tcctgggggc cccagccaag
      961 cctccgctgg tcagggccga catggtgaca gagaagcagg cgcgggagct gagtgtgcct
     1021 cagggtcctg gggctggagc agaaagcact ggagaaatca tcaacaacac tgtgcccttg
     1081 gagaacagca ttcctgggaa ctgctgctct gccctgttca agaacctgct tctcaagaag
     1141 atcaagcggt gtgagcggaa gggcactgag tctgtcacag aggagaagtg cgctgtgctc
     1201 ttctctgcca gcttcacact tggccccggc aaactcccca tccagctcca ggccctgtct
     1261 ctgcccctgg tggtcatcgt ccatggcaac caagacaaca atgccaaagc cactatcctg
     1321 tgggacaatg ccttctctga gatggaccgc gtgccctttg tggtggctga gcgggtgccc
     1381 tgggagaaga tgtgtgaaac tctgaacctg aagttcatgg ctgaggtggg gaccaaccgg
     1441 gggctgctcc cagagcactt cctcttcctg gcccagaaga tcttcaatga caacagcctc
     1501 agtatggagg ccttccagca ccgttctgtg tcctggtcgc agttcaacaa ggagatcctg
     1561 ctgggccgtg gcttcacctt ttggcagtgg tttgatggtg tcctggacct caccaaacgc
     1621 tgtctccgga gctactggtc tgaccggctg atcattggct tcatcagcaa acagtacgtt
     1681 actagccttc ttctcaatga gcccgacgga acctttctcc tccgcttcag cgactcagag
     1741 attgggggca tcaccattgc ccatgtcatc cggggccagg atggctctcc acagatagag
     1801 aacatccagc cattctctgc caaagacctg tccattcgct cactggggga ccgaatccgg
     1861 gatcttgctc agctcaaaaa tctctatccc aagaagccca aggatgaggc tttccggagc
     1921 cactacaagc ctgaacagat gggtaaggat ggcaggggtt atgtcccagc taccatcaag
     1981 atgaccgtgg aaagggacca accacttcct accccagagc tccagatgcc taccatggtg
     2041 ccttcttatg accttggaat ggcccctgat tcctccatga gcatgcagct tggcccagat
     2101 atggtgcccc aggtgtaccc accacactct cactccatcc ccccgtatca aggcctctcc
     2161 ccagaagaat cagtcaacgt gttgtcagcc ttccaggagc ctcacctgca gatgcccccc
     2221 agcctgggcc agatgagcct gccctttgac cagcctcacc cccagggcct gctgccgtgc
     2281 cagcctcagg agcatgctgt gtccagccct gaccccctgc tctgctcaga tgtgaccatg
     2341 gtggaagaca gctgcctgag ccagccagtg acagcgtttc ctcagggcac ttggattggt
     2401 gaagacatat tccctcctct gctgcctccc actgaacagg acctcactaa gcttctcctg
     2461 gaggggcaag gggagtcggg gggagggtcc ttgggggcac agcccctcct gcagccctcc
     2521 cactatgggc aatctgggat ctcaatgtcc cacatggacc taagggccaa ccccagttgg
     2581 tagaacccag ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     2641 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2701 tggctttata tatcttgtgg aaaggacgat agtgacaggc cacattacta tgt
//
