LOCUS       sequence-286065-        1496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3174
FEATURES             Location/Qualifiers
     source          1..1496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      383..392
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1328..1352)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1360..1401
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctaaggagaa caggggggat
       61 gatggggcca ccgagaggcc gaagaaggcc aaggaggagc ggcgcatgga ccagatgatg
      121 ccaggaaaga ccaaagcaga tgctgctgac ccagcaccac ttcccagcca ggagcccccc
      181 aggaacagca cagaacagca gggcctggcc tctgggtctt ctgtgtcctt tgtgggtgcc
      241 agcggctgtc ctgaggccta caagcggctc ctatccagct tctactgcaa ggggacacac
      301 ggcatcatgt caccactggc caaaaagaag ctcctggccc aggtgagcaa ggtggaggcc
      361 ttgcagtgcc aggaggaggg ctgccgccat ggggcagagc cccaggcgtc cccagctgtt
      421 cacctcccag agagtcccca gagccccaaa gggctgactg agaactccag gcaccggctg
      481 acccctcagg agggattgca ggccccaggt ggcagcctca gagaggaggc gcaggcaggc
      541 ccctgcccgg cagcccccat cttcaagggc tgcttctaca cccaccccac cgaggtgctg
      601 aagcctgtca gccagcaccc cagggacttc ttctctagac ttaaagatgg ggtgctattg
      661 gggcctcctg gcaaagaggg gctgtcagtg aaagagcccc agctggtgtg gggcggagac
      721 gctaaccgcc cttctgcgtt ccataaaggt ggctccagaa agggcatcct ctaccccaag
      781 cccaaagcct gctgggtgtc ccccatggcc aaggtcccag ccgagagccc cacgctcccg
      841 cccaccttcc ccagtagccc aggcctgggc agcaagcgca gcctggagga agagggtgct
      901 gcccacagtg ggaagagact gcgggccgtg tctccctttc ttaaggaggc ggatgccaag
      961 aagtgtgggg ccaaacctgc agggtccggc ctggtctcct gccttctggg cccagccctg
     1021 gggcctgtgc ccccagaggc ctacaggggc accatgctgc actgcccgct gaacttcact
     1081 ggcaccccgg gccccttgaa gggccaggct gcactcccct tcagccccct ggtcatcccg
     1141 gccttcccgg cccacttcct ggccaccgca ggcccctcgc ccatggccgc tggcctgatg
     1201 cacttccccc caacgtcctt cgacagtgcc ctccgccaca gactttgccc ggcctcatct
     1261 gcctggcacg caccaccagt cacaacctat gcagcgcccc acttcttcca cctcaacacc
     1321 aagctgtacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1381 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1441 tcttggcttt atatatcttg tggaaaggac gactgagtta cgataagtaa gcacac
//
