LOCUS       sequence-286071-        2351 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3176
FEATURES             Location/Qualifiers
     source          1..2351
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2183..2207)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2215..2256
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aagcacgttc tatgctggtt
       61 ccaccccagg catctgtgtg cttcgaggat gtggctatgg cattcacaca ggaggagtgg
      121 gaacagctgg acctggccca gaggacactg taccgagagg tgacactgga gacctgggag
      181 catattgtct ccctggggct tttcctttcc aaatctgatg tgatctctca gctggagcaa
      241 gaagaggacc tgtgcagggc agagcaggag gccccccgag actggaaagc tacccttgag
      301 gagaataggt tgaattctga aaaagatcga gctagggaag aactatccca ccacgtggaa
      361 gtgtacagga gtggaccgga ggagccaccc tctttggtat taggaaaagt gcaagatcag
      421 agcaaccagt taagggaaca ccaggagaac tccttgaggt tcatggtact cacctcagag
      481 agactgtttg ctcaaaggga acattgtgag cttgaacttg ggggaggtta ttctctacct
      541 tctactttaa gccttctacc tacaacatta cctacaagta caggtttccc taagcccaac
      601 tcacaagtta aagagttgaa acaaaattca gctttcatta atcatgagaa aaatggagca
      661 gatgggaagc actgtgagag tcatcagtgt gctagagctt tctgtcagag tatttacttg
      721 agtaaacttg gaaacgttga aacaggaaag aaaaaccctt atgaatatat tgtcagtggt
      781 gactctctca actatggttc ctccctttgt tttcatggta gaactttttc agtgaagaaa
      841 agtgatgact gtaaggatta tggaaacctc ttcagtcaca gtgtgtctct gaatgaacag
      901 aagccagtgc attttgggaa aagtcagtat gagtgtgatg agtgcaggga aacctgttct
      961 gagagtctgt gccttgtaca aacagaaaga agtggccctg gagagacccc cttcagatgt
     1021 gaggaacgct gtgctgcctt ccccatggcc tcatcttttt ctgactgtaa catcattcag
     1081 actacagaga agccatctgt gtgtaatcag tgtggaaaat ctttcagctg ttgtaagctc
     1141 atacaccaga gaacacacac tggagaaaag cccttcgaat gtactcagtg tgggaaatct
     1201 tttagccaga gctatgacct tgtcatacat cagaggacac acactggaga gaagccctat
     1261 gagtgtgacc tgtgtgggaa atccttcacc cagagatcca aacttattac acatcagcga
     1321 attcacactg gagaaaaacc gtatcagtgt attgaatgca gaaaatcctt caggtggaac
     1381 tctaacctca ttgtacatca gagaattcat actggagaga aaccgtatga gtgcactcac
     1441 tgtggaaagt ccttcagcca aagctatgag ttagttacac ataaaagaac gcacactgga
     1501 gaaaaaccct tcaaatgtac tcagtgtggg aaatctttca gccagaagta tgaccttgtt
     1561 gtacatcaga ggacacacac tggagagaag ccctatgagt gcaacctgtg tgggaaatcc
     1621 ttctcccaga gttccaaact tattacgcat cagcgaattc acactggaga aaaaccgtat
     1681 cagtgtattg aatgtgggaa atccttcaga tggaactcta acctcgtcat acatcagaga
     1741 attcatactg gagagaaacc gtacgattgc actcactgtg gaaagtcctt cagccaaagc
     1801 tatcagttag ttgcacataa aagaactcac actggagaaa agccctatga atgtaacgag
     1861 tgtggaaaag ccttcaatcg aagcactcag ctcatcaggc atctgcaaat tcacactggg
     1921 gagaagccgt acaaatgcaa tcagtgcaat aaagcctttg caaggagctc ctaccttgtg
     1981 atgcatcaga gaactcacac tggtgagaaa ccttttgagt gtagtcagtg tgggaaagcc
     2041 ttttcaggga gctctaacct tctttcccat cacagaattc attctggaga gaaaccctat
     2101 gaatgtagtg actgtgggaa atccttccgg cagcaatctc aacttgtagt gcatcggcgg
     2161 acacatactg gagagaaacc ttacccaact ttcttgtaca aagtggttga tatcggtaag
     2221 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     2281 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacgc tcaccagcag
     2341 tccttaggct t
//
