LOCUS       sequence-286077-        1364 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3178
FEATURES             Location/Qualifiers
     source          1..1364
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1196..1220)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1228..1269
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga ttataagcac accacagaga
       61 ctaaccagtt caggaagtgt tctgattggg agtccatata cccctgcacc agcaatggtt
      121 actcagacac acatagcaga agctactggc tgggtccctg gtgatagaaa acgggctaga
      181 aaatttatag actctgattt ttcagaaagt aaacgaagca aaaaaggaga taaaaatggg
      241 aaaggcttga gacacttttc aatgaaagtg tgtgagaaag ttcaacgaaa aggtacaaca
      301 tcgtacaatg aagtcgctga tgagctggtg tcagagttca ccaattcaaa taaccatttg
      361 gctgctgatt cgcaggctta tgatcagaag aacattaggc gaagagttta tgatgcttta
      421 aatgtgctaa tggcaatgaa cataatttca aaggaaaaaa aagaaatcaa gtggattggc
      481 ctgcctacca attctgctca ggaatgtcag aatctggaga tagagaagca gaggcggata
      541 gaacggataa agcagaagcg ggcccagctg caagaacttc tcctacagca aatcgctttc
      601 aaaaacctgg tacagagaaa tcgacaaaat gagcagcaaa accagggccc gccggctctg
      661 aactctacca ttcagctgcc attcataatc atcaatacaa gcagaaaaac agtcatagat
      721 tgcagcatct ccagtgacaa gtttgagtat cttttcaatt ttgacaacac ctttgagatc
      781 catgatgaca tagaagtact aaagcggatg ggaatgtcgt ttggcctgga gtcaggcaaa
      841 tgctctctgg aggatctgaa acttgcgaaa tccctggtgc caaaggcttt agaaggttat
      901 atcacagata tctccacagg accttcttgg ttaaatcagg gactacttct gaactctacc
      961 caatcagttt caaatttaga cctgaccact ggtgccacct taccccagtc aagtgtaaac
     1021 caagggttat gcttggatgc agaagtggcc ttagcaactg ggcagttcct ggccccaaac
     1081 agtcaccagt ccagcagtgc ggcctctcac tgctccgagt cccgaggcga gaccccctgt
     1141 tcgttcaatg atgaagatga ggaagatgat gaggaggatt cctcctcccc agaataccca
     1201 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     1261 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1321 atatcttgtg gaaaggacga atcacggcat ttaaggagtg gggt
//
