LOCUS       sequence-286080-        1400 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3179
FEATURES             Location/Qualifiers
     source          1..1400
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1232..1256)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1264..1305
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggaaatagt ggggtgccga
       61 gcagaagaca actcgtgtcc tttccgcccc ccagccatgc tctttcacgg gatctccgga
      121 ggccacatcc aaggcatcat ggaggagatg gagcgcagat ccaagactga ggcccgtctg
      181 gccaaaggcg cccagctcaa cggccgcgac gcgggcatgc ccccgctcag cccggagaag
      241 cccgccctgt gcgccggctg cgggggcaag atctcggaca ggtactatct gctggctgtg
      301 gacaaacagt ggcatctgag atgcctgaag tgctgtgaat gtaagctggc cctcgagtcc
      361 gagctcacct gctttgccaa ggacggtagc atttactgca aggaggatta ctacagaagg
      421 ttctctgtgc agagatgtgc ccgctgccac cttggcattt ccgcctcgga gatggtcatg
      481 cgcgcccgag actctgtcta ccacctgagc tgcttcacct gctccacttg caacaagact
      541 ctgaccacgg gcgaccattt cggcatgaag gacagcctgg tgtactgccg cgcccacttc
      601 gagaccctct tgcaaggaga gtatccaccg cagctgagct acacggagct ggcggccaag
      661 agcggcggcc tggccctgcc ttacttcaac ggtacgggca ccgtgcagaa agggcggccc
      721 cggaagcgga agagcccagc gctgggagtg gacatcgtca attacaactc aggttgtaat
      781 gagaatgagg cagaccactt ggaccgggac cagcagcctt atccaccctc gcagaagacc
      841 aagcgcatgc gaacctcttt caagcatcac cagctccgga ccatgaaatc ctactttgcc
      901 atcaaccaca acccggatgc caaggacctc aagcagcttg cccagaaaac aggtctgacc
      961 aaaagagttt tgcaggtttg gttccaaaac gcacgagcca aattcagaag gaaccttttg
     1021 cggcaggaga atgggggtgt tgataaagct gacggcacgt cgcttccggc cccgccctca
     1081 gcagacagcg gagctctcac tccacccggc actgcgacca ctttaacaga cctgaccaat
     1141 cccactatca ctgtagtgac atccgtgacc tctaacatgg acagccacga atccggaagc
     1201 ccctcacaaa ctaccttaac aaaccttttc ttgccaactt tcttgtacaa agtggttgat
     1261 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1321 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgatacc
     1381 ggacatgctc actgtgcgaa
//
