LOCUS       sequence-286086-        1817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3181
FEATURES             Location/Qualifiers
     source          1..1817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      743..752
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1649..1673)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1681..1722
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agcgggtgaa cgacgcttcg
       61 tgcggcccgt ctggctgcta cacataccag gtgagcagac acagcacgga gatgctgcac
      121 aacctgaacc agcagcgcaa aaacggcggg cgcttctgcg acgtactctt gcgggtaggc
      181 gacgagagct tcccagcgca ccgcgccgtg ctggccgcct gcagcgagta ctttgagtcg
      241 gtgttcagcg cccagttggg cgacggcgga gctgcggacg ggggtccggc tgatgtaggg
      301 ggcgcgacgg cagcaccagg cggcggggcc gggggcagcc gggagctgga gatgcacact
      361 atcagctcca aggtatttgg ggacattctg gacttcgcct acacttcccg catcgtggtg
      421 cgcttggaga gctttcccga actcatgacg gccgccaagt tcctgctgat gaggtcggtt
      481 atcgagatct gccaggaagt catcaaacag tccaacgtac agatcctggt accccctgcc
      541 cgcgccgata taatgctctt tcgcccccct gggacctcgg acttgggctt ccctttggac
      601 atgaccaacg gggcagcctt ggcagccaac agcaatggca tcgccggcag catgcagcca
      661 gaggaggagg cagctcgggc ggctggtgca gccattgcag gccaagcctc tttgcctgtg
      721 ttacctgggg tggaccgctt gcccatggtg gctggacccc tatcccccca actgctgact
      781 tccccattcc ccagtgtggc atccagtgcc cctcccctga ctggcaagcg aggccggggc
      841 cgcccaagga aggccaacct gctggactca atgtttgggt ccccaggggg cctgagggag
      901 gcaggcatcc ttccatgcgg tctatgtggt aaggtgttca ctgatgccaa ccggctccgg
      961 cagcacgagg cccagcacgg tgtcaccagc ctccagctgg gctacatcga ccttcctcct
     1021 ccgaggctgg gtgagaatgg gctacccatc tctgaagacc ccgacggccc ccgaaagagg
     1081 agccggacca ggaagcaggt ggcttgtgag atctgcggca agatcttccg tgatgtgtat
     1141 catcttaacc ggcacaagct gtcccactct ggggagaagc cctactcctg ccctgtgtgt
     1201 gggttgcggt tcaagagaaa agaccgcatg tcctaccatg tgcggtccca tgatgggtcc
     1261 gtgggcaagc cttacatctg ccagagctgt gggaaaggct tctccaggcc tgatcacttg
     1321 aacggacata tcaagcaggt gcacacttct gagcggcctc acaagtgtca ggtgtgggtt
     1381 gggagcagca gcggcctgcc gcccctggaa cctcttccta gcgacctgcc atcatgggac
     1441 tttgcccagc ctgctttgtg gaggtcgtcc cattcggttc ctgacaccgc cttttccctt
     1501 tctctaaaaa aatcattccc agcccttgaa aacctgggcc cagcacactc cagcaacact
     1561 ctcttctgcc cagccccgcc gggatatctg aggcagggct ggaccacccc agagggcagc
     1621 agggccttta cccagtggcc tgttggctac ccaactttct tgtacaaagt ggttgatatc
     1681 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1741 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatgcatta
     1801 gagctaatct acgcgcg
//
