LOCUS       sequence-286089-        1841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3182
FEATURES             Location/Qualifiers
     source          1..1841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1673..1697)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1705..1746
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaccttcga ggatgtggcc
       61 gtgtacttct cccaggagga gtgggggctc ctggacacag cgcagagggc cctgtaccgc
      121 cacgtgatgc tggaaaactt cacacttgtg acctcacttg gactctctac ctcccgacct
      181 cgtgtggtca ttcaacttga gcgtggcgag gagccctggg ttcccagtgg aaaggacatg
      241 accctggcca ggaacaccta cgggaggctc aactctggtt cctggagttt gacagaggat
      301 agagatgttt ctggagaatg gccacgagct ttcccagata ccccacctgg gatgactact
      361 agcgtcttcc cagttgccga tgcctgccac agtgtaaaaa gcctgcagcg acaaccgggt
      421 gcctccccat ctcaggagag aaaacccacg ggggtgtcgg tgatctactg ggagaggctc
      481 ctgctaggct cgcgcagtga ccaggccagc atcagcctgc gactgacctc cccactcagg
      541 ccccccaaga gcagccggcc cagggaaaag accttcacag agtaccgggt gcctgggagg
      601 cagcccagga cgcctgagcg gcagaagcca tgtgcacagg aggtccctgg gagagccttc
      661 gggaatgcct cggacctgaa ggccgccagt ggtggcaggg atcgcagaat gggcgcagct
      721 tggcaggagc ctcatagact cctcggtggc caggagccct cgacctggga cgagctgggc
      781 gaggctcttc acgctgggga gaagtccttc gaatgcaggg cgtgcagcaa agtgttcgtg
      841 aagagctccg acctcctcaa gcacctacgc acccacaccg gggagcggcc ctacgagtgc
      901 acccagtgcg gcaaggcctt cagccagacg tcgcacttga cgcagcacca gcgcatccac
      961 agcggcgaga cgccctacgc gtgccccgtg tgcggcaagg ccttccggca tagctcctcg
     1021 ctggtgcggc accagcgcat ccacacggcc gagaagtcct tccgctgctc cgagtgcggc
     1081 aaggccttca gccacggctc caacctcagc cagcaccgca agatccacgc gggtgggcgt
     1141 ccttatgctt gcgcacagtg tggccgccgc ttctgccgca actcgcacct gatccagcac
     1201 gagcgtacgc acacaggcga gaagcccttc gtatgcgcgc tctgcggtgc tgccttcagc
     1261 cagggctcct cgctcttttt gcaccagcgc gtgcacacag gcgagaagcc cttcgcctgc
     1321 gcccagtgcg gccgctcctt tagccgcagc tccaacctca cccagcacca gctcctgcac
     1381 acgggcgagc ggcccttccg ctgcgtggac tgtggcaagg gtttcgccaa gggcgccgtg
     1441 ctgctcagcc accggcgcat tcacacgggc gagaagccct tcgtgtgcac gcagtgtggc
     1501 cgcgccttcc gtgagcgccc tgccctcttg caccaccaga ggatccacac cacagagaag
     1561 accaatgccg cagcaccaga ctgcaccccg gggccaggtt tccttcaggg acatcatcgg
     1621 aaggtgcgcc ggggagggaa gccaagccca gtcctgaagc cagcgaaggt cttgccaact
     1681 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1741 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1801 tcttgtggaa aggacgacgt cgagcgtctg gccgtcccat a
//
