LOCUS       sequence-286092-        1763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3183
FEATURES             Location/Qualifiers
     source          1..1763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1595..1619)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1627..1668
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cttcctcccc ggcggtggac
       61 gtgtcctgca ggcggcggga gaagcggcgg cagctggacg cgcgccgcag caagtgccgc
      121 atccgcctgg gcggccacat ggagcagtgg tgcctcctca aggagcggct gggcttctcc
      181 ctgcactcgc agctcgccaa gttcctgttg gaccggtaca cttcttcagg ctgtgtcctc
      241 tgtgcaggtc ctgagccttt gcctccaaaa ggtctgcagt atctggtgct cttgtctcat
      301 gcccacagcc gagagtgcag cctggtgccc gggcttcggg ggcctggcgg ccaagatggg
      361 gggcttgtgt gggagtgctc agcaggccat accttctcct ggggaccctc tttgagccct
      421 acaccttcag aggcacccaa gccagcctcc cttccacata ctactcggag aagttggtgt
      481 tccgaggcca cgagtgggca ggagcttgca gatttggaat ctgagcatga tgagaggact
      541 caagaggcca ggttgcccag gagggtggga cccccaccag agaccttccc acctccagga
      601 gaggaagagg gtgaggaaga agaggacaat gatgaggatg aagaggagat gctcagtgat
      661 gccagcttat ggacctacag ctcctcccca gatgatagtg agcctgatgc ccccagacta
      721 ctgccttccc ctgtcacctg cacacctaaa gagggggaga caccaccagc ccctgcagca
      781 ctctccagtc ctcttgctgt gccggccttg tcagcatcct cattgagttc cagagctcct
      841 ccacctgcag aagtcagggt gcagccacag ctcagcagga cccctcaagc ggcccagcag
      901 actgaggccc tggccagcac tgggagtcag gcccagtctg ctccaacccc ggcctgggat
      961 gaggacactg cacaaattgg ccccaagaga attaggaaag ctgccaaaag agagctgatg
     1021 ccttgtgact tccctggctg tggaaggatc ttctccaacc ggcagtattt gaatcaccac
     1081 aaaaagtacc agcacatcca ccagaagtct ttctcctgcc cagagccagc ctgtgggaag
     1141 tctttcaact ttaagaaaca cctgaaggag cacatgaagc tgcacagtga cacccgggac
     1201 tacatctgtg agttctgcgc ccggtctttc cgcactagca gcaaccttgt catccacaga
     1261 cgtatccaca ctggagaaaa acccctgcag tgtgagatat gcgggtttac ctgccgccag
     1321 aaggcttccc tgaactggca ccagcgcaag catgcagaga cggtggctgc cttgcgcttc
     1381 ccctgtgaat tctgcggcaa gcgctttgag aagccagaca gtgttgcagc ccaccgtagc
     1441 aaaagtcacc cagccctgct tctagcccct caagagtcac ccagtggtcc cctagagccc
     1501 tgtcccagca tctctgcccc tgggcctctg ggatccagcg aggggtccag gccctctgca
     1561 tctcctcagg ctccaaccct gcttcctcag caatgcccaa ctttcttgta caaagtggtt
     1621 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1681 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     1741 gatagcctgc ctcaagtcaa att
//
