LOCUS       sequence-286098-        1598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3185
FEATURES             Location/Qualifiers
     source          1..1598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1430..1454)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1462..1503
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gtgacggaaa cccagagctc
       61 ctgtcaacca gccagaccta caacggccag agcgagaaca acgaagacta tgagatcccc
      121 ccgataacac ctcccaacct cccggagcca tccctcctgc acctggggga ccacgaagcc
      181 agctaccact cgctgtgcca cggcctcacc cccaacggtc tgctccctgc ctactcctat
      241 caggccatgg acctcccagc catcatggtg tccaacatgc tagcacagga cagccacctg
      301 ctgtcgggcc agctgcccac gatccaggag atggtccact cggaagtggc tgcctatgac
      361 tcgggccggc ccgggcccct gctgggtcgc ccggcaatgc tggccagcca catgagtgcc
      421 ctcagccagt cccagctcat ctcgcagatg ggcatccgga gcagcatcgc ccacagctcc
      481 ccatcaccgc cggggagcaa gtcagcgacc ccctctccct ccagctccac tcaggaagag
      541 gagtcggaag tgcatttcaa gatctcggga gaaaagagac cttcagccga cccaggaaaa
      601 aaggccaaga acccgaagaa gaagaaaaag aaggacccca atgagccgca gaagcctgtg
      661 tcggcctacg cactcttctt cagagacact caggccgcca tcaagggtca gaaccccagt
      721 gccactttcg gtgacgtgtc caaaatcgtg gcctccatgt gggacagcct gggagaggaa
      781 cagaagcagg cctacaagag gaagacagaa gcagcaaaga aggaatatct gaaggccctg
      841 gcagcctacc gggctagcct cgtctccaag agctccccag atcaaggtga gaccaagagc
      901 actcaggcaa acccaccagc caaaatgctc ccacccaagc agcccatgta tgccatgcca
      961 ggcctggcct ccttcctgac gccgtcggac ctgcaggcct tccgcagtgg ggcctcccct
     1021 gccagcctcg cccggacgct gggctccaag tctctgctgc caggcctcag tgcgtccccg
     1081 ccgccgccac cctccttccc gctcagcccc acactgcacc agcagctgtc actgccccct
     1141 cacgcccagg gcgccctcct cagtccacct gttagcatgt ccccagcccc ccagccccct
     1201 gtcctgccca cccccatggc actccaggtg cagctggcga tgagcccctc acctccaggg
     1261 ccacaggact tcccgcacat ctctgagttc cccagcagct cgggatcctg ctcacctggc
     1321 ccatccaacc ccaccagcag cggggactgg gacagcagct accccagtgg ggagtgtggc
     1381 atcagcacct gcagcctgct ccccagggac aaatcgctct acctcaccta cccaactttc
     1441 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1501 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1561 tgtggaaagg acgaattata aggaatgtct acaaaagg
//
