LOCUS       sequence-286101-        1130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3186
FEATURES             Location/Qualifiers
     source          1..1130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(962..986)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             994..1035
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg tttcagacga agatgaattg
       61 aatcttctgg ttattgtagt tgatgccaac ccaatttggt ggggaaagca agcattaaag
      121 gaatctcagt tcactttatc caaatgcata gatgccgtga tggtgctggg aaattcgcat
      181 ttattcatga atcgttccaa caaacttgct gtgatagcaa gtcacattca agaaagccga
      241 ttcttatatc ctggaaagaa tggcagactt ggagacttct tcggagaccc tggcaaccct
      301 cctgaattta atccctctgg gagtaaagat ggaaaatacg aacttttaac ctcagcaaat
      361 gaagttattg ttgaagagat taaagatcta atgaccaaaa gtgacataaa gggtcaacat
      421 acagaaactt tgctggcagg atccctggcc aaagcccttt gctacattca tagaatgaac
      481 aaggaagtta aagacaatca ggaaatgaaa tcaaggatat tggtgattaa ggctgcagaa
      541 gacagtgcgt tgcagtatat gaacttcatg aatgtcatct ttgcagcaca gaaacagaat
      601 attttgattg atgcctgtgt tttagactcc gactcagggc tcctccaaca ggcttgtgac
      661 atcacgggag gactgtacct gaaggtgcct cagatgcctt ctcttctgca gtatttgctg
      721 tgggtgtttc ttcccgatca agatcagaga tctcagttaa tcctcccacc cccggttcat
      781 gttgactaca gggctgcttg cttctgtcat cgaaatctca ttgaaattgg ttatgtctgt
      841 tctgtgtgtt tgtcaatatt ctgcaatttc agccccattt gtactacgtg cgagacagcc
      901 tttaaaattt ctctgcctcc agtgctgaaa gccaagaaaa agaaactgaa agtgtctgcc
      961 tgcccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1021 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1081 ctttatatat cttgtggaaa ggacgatagc cactgagatg gggtcgacac
//
