LOCUS       sequence-286104-        2099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3187
FEATURES             Location/Qualifiers
     source          1..2099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1931..1955)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1963..2004
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt tctacgcaca ttttgttctc
       61 agtaaaagag ggcctctggc caaaatttgg ctagcggccc attgggataa gaagctaacc
      121 aaagcccatg tgttcgagtg taatttagag agcagcgtgg agagtatcat ctcaccaaag
      181 gtgaaaatgg cattacggac atcaggacat ctcttactgg gagtagttcg aatctatcac
      241 aggaaagcca aataccttct tgcagactgt aatgaagcat tcattaagat aaagatggct
      301 tttcggccag gtgtggttga cctgcctgag gaaaatcggg aagcagctta taatgccatt
      361 actttacctg aagaatttca tgactttgat cagccactgc ctgacttaga tgacatcgat
      421 gtggcccagc agttcagctt gaatcagagt agagtggaag agataaccat gagagaagaa
      481 gttgggaaca tcagtatttt acaagaaaat gattttggtg attttggaat ggatgatcgt
      541 gagataatga gagaaggcag tgcttttgag gatgacgaca tgttagtaag cactactact
      601 tctaacctcc tattagagtc tgaacagagc accagcaatc tgaatgagaa aattaaccat
      661 ttagaatatg aagatcaata taaggatgat aattttggag aaggaaatga tggtggaata
      721 ttagatgaca aacttattag taataatgat ggcggtatct ttgatgatcc ccctgccctc
      781 tctgaggcag gggtgatgtt gccagagcag cctgcacatg acgatatgga tgaggatgat
      841 aatgtatcaa tgggtgggcc tgatagtcct gattcagtgg atcccgttga accaatgcca
      901 accatgactg atcaaacaac acttgttcca aatgaggaag aagcatttgc attggagcct
      961 attgatataa ctgttaaaga aacaaaagcc aagaggaaga ggaagctaat tgttgacagt
     1021 gtcaaagagt tggatagcaa gacaattaga gcccaactta gtgattattc agatattgtt
     1081 actactttgg atctggcacc gcccaccaag aaattgatga tgtggaaaga gacaggagga
     1141 gtagaaaaac tgttttcttt acctgctcag cctttgtgga ataacagact actgaagctc
     1201 tttacacgct gtcttacacc gcttgtacca gaagacctta gaaaaaggag gaaaggagga
     1261 gaggcagata atttggatga attcctcaaa gaatttgaaa atccagaggt tcctagagag
     1321 gaccagcaac agcagcatca gcagcgtgat gttatcgatg agcccattat tgaagagcca
     1381 agccgcctcc aggagtcagt gatggaggcc agcagaacaa acatagatga gtcagctatg
     1441 cctccaccac cacctcaggg agttaagcga aaagctggac aaattgaccc agagcctgtg
     1501 atgcctcctc agcaggtaga gcagatggaa ataccacctg tagagcttcc cccagaagaa
     1561 cctccaaata tctgtcagct aataccagag ttagaacttc tgccagaaaa agagaaggag
     1621 aaagagaagg aaaaagaaga tgatgaagag gaagaggatg aagatgcatc agggggcgat
     1681 caagatcagg aagaaagaag atggaacaaa aggactcagc agatgcttca tggtcttcag
     1741 cgtgctcttg ctaaaactgg agctgaatct atcagtttgc ttgagttatg tcgaaatacg
     1801 aacagaaaac aagctgccgc aaagttctac agcttcttgg ttcttaaaaa gcagcaagct
     1861 attgagctga cacaggaaga accgtacagt gacatcatcg caacacctgg accaaggttc
     1921 catattatat acccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1981 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     2041 atttcttggc tttatatatc ttgtggaaag gacgattctt acacataaca atggacaat
//
