LOCUS       sequence-286107-        1889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3188
FEATURES             Location/Qualifiers
     source          1..1889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1721..1745)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1753..1794
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga attccagctt gaccgcccag
       61 aggcgcggca gtgacgccga gttgggaccc tgggtgatgg ctgcgaggtc caaggacgcg
      121 gcgccgtccc aacgcgacgg acttttgccc gtgaaagtgg aggaagactc acccggaagt
      181 tgggagccca actatcccgc ggcttcgccg gaccccgaaa cttctcgact gcactttagg
      241 cagctgcgtt accaggaggt ggctggaccg gaagaggcgc tgagccggct ccgagaactc
      301 tgtcgtcggt ggctgagacc cgagctgctc tccaaggagc agatcctgga gctgctggtg
      361 ctggagcagt tcctcaccat cctgcccgag gagcttcaag cctgggtgcg agagcactgc
      421 ccagagagcg gggaggaggc ggtggccgtg gtgcgggctc tgcagcgagc gctcgatgga
      481 acctcatccc aggggatggt gactttcgag gacacggctg tgtctctaac ctgggaggag
      541 tgggagcgcc tggacccagc acggagggac ttctgcagag agagtgcgca gaaggattcc
      601 gggagcacag ttccgccgag tttggaaagc agagtggaga acaaagagtt gattccaatg
      661 caacaaattt tagaagaagc ggagccacag gggcaactac aagaagcgtt ccaggggaag
      721 cgccccctgt tttctaagtg tggcagtacc catgaggaca gggtggaaaa gcagtccgga
      781 gaccccttgc ccctgaaact tgaaaattct cctgaagcag aaggactcaa cagcatctca
      841 gatgtcaata agaatggttc catagaaggg gaagactcta aaaataatga attgcagaac
      901 agtgccaggt gttccaacct tgttctatgt cagcacatcc cgaaagcaga gaggcccact
      961 gacagtgagg aacacgggaa caagtgcaag caaagtttcc acatggtgac gtggcacgtg
     1021 ctgaaacctc acaagtctga cagtggagac agtttccatc attccagcct ttttgagacc
     1081 cagaggcagc tccatgaaga aagaccttat aaatgtggta actgtgggaa gagtttcaaa
     1141 caacgctctg acctctttag acaccagaga atccacacag gtgagaaacc ctatggctgc
     1201 caagaatgtg ggaaaagctt cagccagagt gctgccctga ccaagcacca gaggacacac
     1261 acaggcgaga agccgtacac ctgtctgaaa tgtggggagc gcttcaggca gaattcacac
     1321 ctaaatcgtc atcaaagtac ccacagtaga gacaaacatt ttaaatgtga ggaatgcggg
     1381 gaaacctgtc atatttccaa cctttttaga catcagagac tacataaagg ggaaagaccc
     1441 tataagtgtg aagaatgcga gaagagcttc aaacagcgct ctgacctctt taaacaccac
     1501 agaatccaca ctggggagaa gccctatgga tgttccgtct gtgggaaacg cttcaatcag
     1561 agtgcaaccc tcattaaaca ccagagaatt cacactgggg aaaagcctta caaatgtctt
     1621 gaatgtgggg aaagatttag acaaagtaca caccttatcc gacaccaaag aattcatcaa
     1681 aataaagtgc tgtcggctgg gcgtggtggc tcacgcctat acccaacttt cttgtacaaa
     1741 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1801 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1861 gacgacgcgg catggcaaga gttcatatg
//
