LOCUS       sequence-286110-        1412 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3189
FEATURES             Location/Qualifiers
     source          1..1412
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1244..1268)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1276..1317
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggctgtcat cctgccctcg
       61 actgctgctc cgtcttccct gttcccagcc tctcagcaaa aaggacacac acagggcgga
      121 gagctggtta atgagctcct gacaagctgg ctacggggct tggtaacctt cgaggatgtg
      181 gccgtggagt tcacccagga ggagtgggcg ttgctggacc ctgcccaaag gacactgtac
      241 agggatgtga tgctggagaa ctgcaggaac ctggcctcac tagggtgtcg tgttaataaa
      301 cccagtctga tatcccagtt ggaacaagac aagaaggtgg tgacagagga aagaggaatt
      361 ctaccaagca cctgtccaga tttggagact ctacttaaag ccaaatggtt aactcctaag
      421 aagaatgttt tcagaaaaga acagtctaaa ggtgtaaaaa cggaaagaag tcatcgtgga
      481 gtgaaactca atgaatgtaa tcagtgtttt aaagtcttca gcacgaaatc taacctaact
      541 cagcacaaga gaattcatac tggagaaaaa ccctatgact gtagtcaatg tgggaagtcc
      601 ttcagtagca gatcttacct tactattcat aagagaatcc ataatgggga gaaaccctat
      661 gaatgcaatc actgtgggaa agcatttagt gatccctcat cccttagact gcatttgaga
      721 attcacactg gagaaaaacc ctatgaatgt aaccagtgtt ttcacgtttt ccgcaccagt
      781 tgtaacctca aaagccacaa gaggattcac acgggggaga atcaccatga atgtaatcag
      841 tgtggaaaag ctttcagcac aaggtcctct ctcactgggc acaatagcat tcatacaggg
      901 gagaaacctt atgaatgtca cgattgtggg aaaaccttca ggaagagctc ctatctgaca
      961 cagcacgtaa gaactcatac tggagaaaaa ccctatgaat gtaacgagtg tgggaaatcc
     1021 ttcagcagta gcttttctct tactgtgcac aagagaatac ataccggaga gaaaccctac
     1081 gagtgcagtg actgtggaaa agcctttaat aatctctcag ctgtgaagaa acacttaaga
     1141 actcacactg gagaaaaacc ctatgaatgt aatcattgtg ggaaatcctt cacaagtaac
     1201 tcctatcttt ctgtgcacaa gagaatacat aatagatgga tatgcccaac tttcttgtac
     1261 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1321 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1381 aaggacgata tcgtggtacg aggaaacttg at
//
