LOCUS       sequence-286113-        1820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3190
FEATURES             Location/Qualifiers
     source          1..1820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1652..1676)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1684..1725
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg tcacctgcgc ccacctgggc
       61 cggcgcgcgc gactcccggc agctcagccc tctgcctgcc caggaacctg cttttcccaa
      121 gaggagagaa tggctgctgg gtacctgccc cgctggtccc aggaattagt aacctttgag
      181 gacgtgtcca tggacttctc ccaggaggag tgggagttgc tggagcctgc tcagaagaac
      241 ctgtacagag aggtgatgct ggagaactac aggaacgtgg tctccctgga agccttgaag
      301 aaccaatgta ctgatgtggg gattaaagag ggtccacttt ccccagcaca aacctcacaa
      361 gtcactagtc tttcctcatg gacggggtat ttactttttc aaccagtggc ttcttcccac
      421 ttggagcaaa gagaagccct gtggatagag gaaaaaggaa ctcctcaagc ctcctgttca
      481 gattggatga ctgtactaag aaaccaagac tcaacttaca agaaggtggc tttgcaggag
      541 gaaccagcca gtggtataaa tatgataaag cttatcagag aagatggggg atggaagcag
      601 ttagaggaca gccatgaaga cccccagggg cttttgagcc aaaaggcatc ccttcacgta
      661 gtggccgttc ctcaggagaa ggctactgca tggcatggat ttggggaaaa tggtaatctg
      721 agcccagccc ttgttttatc acagggaagc tctaaaggga accacttgtg tggcagcgag
      781 ttagatatta caagcttggc atccgattca gtcttaaacc accatcagct gggatatgca
      841 gatcggagac cttgtgaaag taatgaatgt ggaaatgcca tccgccagaa cagtcacttt
      901 attcaacacg gggggaagat gtttgtgtat ttggaaaatg ggcagtcatt gaaccacggt
      961 atggccctga ctatccacaa caaaatcaac acggcagaga aaccctttga gtgccaccag
     1021 tgtgggaagg tgttcaaccg gaggcattct ttgagcgaac atcaaagaat tcacacgggg
     1081 gagaaaccct acgagtgtca ggagtgtggg cgagccttta cgcacagctc caccctcacg
     1141 cgccatctga gaactcatac tggagagaag ccctacgggt gcggtgagtg cgggaaagcc
     1201 ttcaacagga tctcatcgct gactcagcat cagaggattc acaccgggga aaagccctat
     1261 aaatgtgaag actgtgggaa atccttctgc cagagctctt acctgatctt gcacaagagg
     1321 acacacaccg gagagaagcc ctacgaatgc agtgaatgtg gaaaggcctt cagtgaccgt
     1381 tcctctctca accagcacga gcgaactcac acgggcgaga acccctatga atgtaagcag
     1441 tgtgggagag ccttcagcca gaggtcttcc cttgtgaggc acgagagaac tcacactgga
     1501 gagaaaccct acaggtgtca ggaatgtggg aaagccttca gccagagctc atcccttgtc
     1561 acacatcaga aaactcacag cagccagaaa acctataaaa tcattgactg cgggaaagcg
     1621 ttctaccaga acagacatct tattggatat tacccaactt tcttgtacaa agtggttgat
     1681 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1741 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaccgt
     1801 cacataacat gtgactcaat
//
