LOCUS       sequence-286119-        1643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3192
FEATURES             Location/Qualifiers
     source          1..1643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1475..1499)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1507..1548
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ccagcactat taaggaagcc
       61 ttatcagttg tgagtgagga ccagtcgttg tttgagtgtg cctacggaac gccacacctg
      121 gctaagacag agatgaccgc gtcctcctcc agcgactatg gacagacttc caagatgagc
      181 ccacgcgtcc ctcagcagga ttggctgtct caacccccag ccagggtcac catcaaaatg
      241 gaatgtaacc ctagccaggt gaatggctca aggaactctc ctgatgaatg cagtgtggcc
      301 aaaggcggga agatggtggg cagcccagac accgttggga tgaactacgg cagctacatg
      361 gaggagaagc acatgccacc cccaaacatg accacgaacg agcgcagagt tatcgtgcca
      421 gcagatccta cgctatggag tacagaccat gtgcggcagt ggctggagtg ggcggtgaaa
      481 gaatatggcc ttccagacgt caacatcttg ttattccaga acatcgatgg gaaggaactg
      541 tgcaagatga ccaaggacga cttccagagg ctcaccccca gctacaatgc cgacatcctt
      601 ctctcacatc tccactacct cagagagact cctcttccac atttgacttc agatgatgtt
      661 gataaagcct tacaaaactc tccacggtta atgcatgcta gaaacacagg gggtgcagct
      721 tttattttcc caaatacttc agtatatcct gaagctacgc aaagaattac aactaggcca
      781 gatttaccat atgagccccc caggagatca gcctggaccg gtcacggcca ccccacgccc
      841 cagtcgaaag ctgctcaacc atctccttcc acagtgccca aaactgaaga ccagcgtcct
      901 cagttagatc cttatcagat tcttggacca acaagtagcc gccttgcaaa tccaggcagt
      961 ggccagatcc agctttggca gttcctcctg gagctcctgt cggacagctc caactccagc
     1021 tgcatcacct gggaaggcac caacggggag ttcaagatga cggatcccga cgaggtggcc
     1081 cggcgctggg gagagcggaa gagcaaaccc aacatgaact acgataagct cagccgcgcc
     1141 ctccgttact actatgacaa gaacatcatg accaaggtcc atgggaagcg ctacgcctac
     1201 aagttcgact tccacgggat cgcccaggcc ctccagcccc accccccgga gtcatctctg
     1261 tacaagtacc cctcagacct cccgtacatg ggctcctatc acgcccaccc acagaagatg
     1321 aactttgtgg cgccccaccc tccagccctc cccgtgacat cttccagttt ttttgctgcc
     1381 ccaaacccat actggaattc accaactggg ggtatatacc ccaacactag gctccccacc
     1441 agccatatgc cttctcatct gggcacttac tactacccaa ctttcttgta caaagtggtt
     1501 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1561 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     1621 ctaaataaat tactaaagga cgc
//
